Lab on a Chip Technology: Biomolecular Separation and Analysis, Vol. 2 Book

List of Contributors v

Preface ix

Part I Biomolecule Separation

1 Two-Dimensional Electrophoresis in a Chip Z. Hugh Fan Champak Das Hong Chen 3

2 Liquid Chromatography in Microfluidic Chips Hernan V. Fuentes Adam T. Woolley 13

3 Design and Fabrication of Microfluidic Devices for Flow-Based Separation of Blood Cells Lance L. Munn Abhishek Jain 29

4 Hydrophoretic Method for Continuous Blood Cell Separation Sungyoung Choi Je-Kyun Park 45

5 Microchip Gel Electrophoresis of DNA with Integrated Whole-Column Detection Roger C. Lo Victor M. Ugaz 57

6 Microscale Blood Separation Technology Jeffrey D. Zahn Sung Yang Akif Ündar Pantelis Athanasiou 69

Part II Analysis and Manipulation Technologies on a Chip

7 Microfluidic Drops as Microreactors Charles N. Baroud 111

8 Optical Sectioning for Microfluidics Yeh-Chan Ahn Zhongping Chen 133

9 Acquisition of Single Cell Data in an Optical Microscope Kristin Sott Emma Eriksson Mattias Goksör 151

10 Elaborating Lab-on-a-Chips for Single-Cell Transcriptome Analysis Nathalie Bontoux Luce Dauphinot Marie-Claude Potier 167

11 Integrated Circuit/Microfluidic Chips for Dielectric Manipulation Thomas P. Hunt David Issadore Keith A. Brown Hakho Lee Robert M. Westervelt 187

12 Microchip-based PCR Amplification Systems Nathaniel C. Cady 207

13 Cell Viability Measurement Using a Portable Photodiode Array Chip Joon Myong Song Ho Taik Kwon 221

14 A Charge-Coupled Device Based Optical Detector for Lab-on-a-chip Keith Herold Avraham Rasooly 239

15 PCR Devices using Glass Substrates Hao Yu Jianhua Qin Bingcheng Lin 249

16 Braille Microfluidics Tommaso F. Bersano-Begey Yoko Kamotani Shuichi Takayama 269

17 MicrofluidicDevices for Single-cell Analysis Yan Chen Jiang F. Zhong 287

Index 297