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Preface.
Part I Principles and Methods.
1 Introduction.
1.1 Crystals and Symmetry.
1.2 Protein Solubility.
1.3 Experimental Techniques.
1.4 Crystallization Screenings.
1.5 High-Throughput Crystallization, Imaging, and Analysis.
References.
2 Experimental Techniques.
2.1 X-Ray Sources.
2.2 Detectors.
2.3 Crystal Mounting and Cooling.
2.4 Data Collection Techniques.
References.
3 Principles of X-Ray Diffraction by a Crystal.
3.1 Rational Mathematical Representation of Waves.
3.2 Principles of X-Ray Diffraction by a Crystal.
References.
4 Diffraction Data Evaluation.
4.1 Introductory Remarks.
4.2 Geometric Principles in the Rotation Technique with Normal Flat Detector.
4.3 Autoindexing of Oscillation Images.
4.4 Beam Divergence, Mosaicity, and Partiality.
4.5 Integration of Diffraction Spots.
4.6 Post-Refinement, Scaling, and Averaging of Diffraction Data.
References.
5 Methods for Solving the Phase Problem.
5.1 Isomorphous Replacement.
5.2 Anomalous Scattering.
5.3 Determination of Heavy-Atom Positions.
5.4 Phase Calculation.
5.5 Patterson Search Methods (Molecular Replacement).
References.
6 Phase Improvement by Density Modification and Phase Combination.
6.1 Introduction.
6.2 Solvent Flattening.
6.3 Histogram Matching.
6.4 Molecular Averaging.
6.5 Sayre’s Equation.
6.6 Atomization.
6.7 Phase Combination.
6.8 Difference Fourier Technique.
References.
7 Model Building and Refinement.
7.1 Model Building.
7.2 Crystallographic Refinement.
7.3 Verification and Accuracy of Structure Determination.
References.
8 Crystal Structure Determination of the Time-Course of Reactions and of Unstable Species.
8.1 Introduction.
8.2 Triggering Methods.
8.3 Trapping Methods.
8.4 Laue Diffraction.
References.
9 Structural Genomics.
9.1 Introduction.
9.2 Target Selection.
9.3 Production of Recombinant Proteins.
9.4 Aspects of Automation.
References.
Part II Practical Examples.
Introductory Remarks.
10 Data Evaluation.
10.1 Autoindexing, Refinement of Cell Parameters, and Reflection Integration.
10.2 Scaling of Intensity Diffraction Data.
10.3 A Complex Example of Space Group Determination.
References.
11 Determination of Anomalous Scatterer or Heavy Atom Positions.
11.1 Application of Direct Methods.
11.2 Vector Verification Methods.
11.3 Comparison of the Results from SnB and RSPS.
References.
12 MIRAS and MAD Phasing with the Program SHARP.
12.1 MAD Phasing with the Program SHARP for 4-BUDH.
References.
13 Molecular Replacement.
13.1 Phase Determination of PKC-iota with Program Molrep.
References.
14 Averaging about Non-Crystallographic Symmetry (NCS) for 4-BUDH.
14.1 Determination of NCS Operators for 4-BUDH.
14.2 Electron Density Map Averaging for 4-BUDH.
References.
15 Model Building and More.
15.1 A Very Personal Short Introduction to the Computer Graphics Modeling Program “O”.
15.2 Introduction of the Four Fe-Sites per Fe–S-Cluster and New SHARP-Phasing for 4-BUDH.
15.3 Crystallographic Refinement and Final Steps.
References.
Subject Index.
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Jim Whitted
reviewed X-Ray Crystallography of Biomacromolecules: A Practical Guide on November 08, 2016
X-Ray Crystallography of Biomacromolecules
Ok, I didn't read it ALL, but it is a fantastic resource for the nitty gritty stuff underpinning our bodies and our health.
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Add X-Ray Crystallography of Biomacromolecules: A Practical Guide, Written by one of the most significant contributors to the progress of protein crystallography, this practical guide contains case studies, a troubleshooting section and pointers on data interpretation. It covers the theory, practice and latest achievemen, X-Ray Crystallography of Biomacromolecules: A Practical Guide to the inventory that you are selling on WonderClubX
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Add X-Ray Crystallography of Biomacromolecules: A Practical Guide, Written by one of the most significant contributors to the progress of protein crystallography, this practical guide contains case studies, a troubleshooting section and pointers on data interpretation. It covers the theory, practice and latest achievemen, X-Ray Crystallography of Biomacromolecules: A Practical Guide to your collection on WonderClub |