Wnt Signaling: Volume 2, Pathway Models Book

Part I. Introduction

Chapter 1. Evolution of the Wnt pathway. Jennifer C Croce and David R McClay

Part II. Dictyostelium

Chapter 2. Dictyostelium development: a prototypic Wnt pathway? Adrian J Harwood

Chapter 3. Monitoring patterns of gene expression in Dictyostelium by b -galactosidase staining. Adrian J Harwood

Chapter 4. Use of the Dictyostelium stalk cell assay to monitor GSK-3 regulation. Adrian J Harwood

Part III. Cnidarians

Chapter 5. Wnt signaling in Cnidarians. Thomas Holstein

Chapter 6. Detecting expression patterns of Wnt pathway components in Nematostella vectensis embryos Shalika Kumburegama, Naveen Wijesena, and Athula H. Wikramanayake

Chapter 7. Detection of expression patterns in Hydra pattern formation. Hans Bode, Tobias Lengfeld, Bert Hombayer and Thomas Holstein

Part IV. elegans

Chapter 8: Analysis of Wnt signaling pathways during C. elegans postembryonic development. Samantha Van Hoffelen and Michael A. Herman

Chapter 9. Wnt signaling during C. Elegans embryonic development. Daniel J Marston, Minna Roh, Amanda J Mikels, Roel Nusse and Bob Goldstein

Part V. Drosophila

Chapter 10. Function of the Wingless signaling pathway in Drosophila. Foster C Gonsalves and Ramanuj DasGupta

Chapter 11. Visualisation of PCP defects in the eye and wing of Drosophila melanogaster Natalia Arbuzova and Helen McNeill

Chapter 12. Wingless signaling in Drosophila eye development. Kevin Legent and Jessica Treisman

Chapter 13. High-throughput RNAi screen in Drosophila. Ramanuj DasGupta and Foster C Gonsalves

Part VI. Sea urchin

Chapter 14. Wnt signaling in early sea urchin development. Shalika Kumburegama and Athula H. Wikramanayake

Chapter 15. Detecting expression patterns of Wnt pathway components in sea urchin embryos using whole mount in situ hybridization. Joanna M. Bince and Athula H. Wikramanayake

Chapter 16. Functional analysis of Wnt signaling in the early sea urchin embryo using mRNA microinjection. Joanna M Bince and Athula H. Wikramanayake

Part VII. Zebrafish

Chapter 17. Wnt signaling mediates diverse developmental processes in zebrafish. Heather Verkade and Joan K Heath

Chapter 18. Determination of mRNA and protein expression patterns in zebrafish. Elizabeth L Christie, Adam C Parslow and Joan K Heath

Chapter 19. Manipulation of Gene Expression during Zebrafish Embryonic Development using Transient Approaches, Benjamin M. Hogan, Heather Verkade, Graham J. Lieschke and Joan K. Heath

Chapter 20. Neural patterning and CNS functions of Wnt in Zebrafish. Richard Dorsky

Part VIII. Xenopus

Chapter 21. Studying Wnt signaling in Xenopus. Stefan Hoppler

Section A: Methods for studying Wnt signaling in Xenopus embryos

Chapter 22. Analysis of gene expression in Xenopus embryos. Danielle L Lavery and Stefan Hoppler

Chapter 23. Detection of nuclear b -catenin in xenopus embryos. Francois Fagotto and Carolyn M Brown

Chapter 24. Transgenic reporter tools tracing endogenous canonical Wnt signaling in Xenopus. Tinneke Denayer, Hong Thi Tran and Kris Vleminckx

Chapter 25. Gain-of-function and Loss-of-function strategies in Xenopus. Danielle L Lavery and Stefan Hoppler

Chapter 26. How the mother can help – studying maternal Wnt signaling by antisense-mediated depletion of maternal mRNAs and the host transfer technique. Adnan Mir and Janet Heasman

Chapter 27. Inducible gene expression in transient transgenic Xenopus embryos. Grant N Wheeler, Danielle L Lavery and Stefan Hoppler

Chapter 28. Wnt-Frizzled interactions in Xenopus. Herbert Steinbeisser and Rajeeb K Swain

Section B: Wnt signaling function in Xenopus development

Chapter 29. Dorsal axis duplication as a functional read-out for Wnt activity. Michael Kuhl and Petra Pandur

Chapter 30. Regulation of Convergent Extension by Non-canonical Wnt signaling in the Xenopus Embryo. Lars F Petersen, Hiromasa Ninomiya and Rudolf Winklbauer

Chapter 31. Frizzled-7 dependent tissue separation in the Xenopus gastrula. Rudolf Winklbauer and Olivia Luu