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List of contributors ix
Preface xiii
Introduction Uma Prabhakar xvii
Considerations while setting up cell-based assays Marian Kelley 1
Development, optimization and validation of cell-based assays - 1 Marielena Mata Thomas Lohr 11
Development, optimization and validation of cell-based assays - 2 Manjula Reddy Uma Prabhakar 25
Whole blood ex vivo stimulation assay development, optimization and validation Manjula Reddy Uma Prabhakar 37
Immunohistochemistry assays in Good Laboratory Practice studies Frank Lynch Steve Bernstein Hector Battifora 49
Flow cytometric cell-based assays: an overview of general applications Cuc Davis Manjula Reddy Thomas Williams Uma Prabhakar 73
T-cell surface markers in human peripheral whole blood using flow cytometry Manjula Reddy Cuc Davis Hugh Davis Charles Pendley Uma Prabhakar 85
Intracellular cytokine detection by flow cytometry Julie G. Wilkinson Carlos A. Aparicio Wade E. Bolton 107
Validating reference samples for comparisonin a regulated ELISPOT assay Magdalena Tary-Lehmann Christina D. Hamm Paul V. Lehmann 127
IFN-[gamma] ELISPOT assay validation Manjula Reddy Jackson Wong Charles Pendley Uma Prabhakar 147
IL-5 ELISPOT assay validation Manjula Reddy Jackson Wong Hugh Davis Charles Pendley Uma Prabhakar 173
Validation of the Cylex technology to measure T and B cell activation capacity in clinical trials Marielena Mata Thomas Lohr Jaymala Patel 193
Development of validated neutralization bioassays Manoj Rajadhyaksha Manjula Reddy Jaime Bald Amy Fraunfelter Persymphonie Miller Marian Kelley Uma Prabhakar 209
Endpoint assays in HIV-1 vaccine trials: functioning in a Good Laboratory Practices environment Patricia D'Souza Josephine H. Cox Guido Ferrari Nina Thapa Kunwar Victoria Polonis Marcella Sarzotti-Kelsoe 239
The future direction of cell-based assays Uma Prabhakar Marian Kelley 277
Index 283
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Add Validation of Cell-Based Assays in the GLP Setting: A Practical Guide, The use of cell-based assays within pharmaceutical and biotechnology companies is driven in large part by the need to evaluate the plethora of drug targets derived from genomics and proteomics. In addition, the potential of biomarkers to facilitate the de, Validation of Cell-Based Assays in the GLP Setting: A Practical Guide to the inventory that you are selling on WonderClubX
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Add Validation of Cell-Based Assays in the GLP Setting: A Practical Guide, The use of cell-based assays within pharmaceutical and biotechnology companies is driven in large part by the need to evaluate the plethora of drug targets derived from genomics and proteomics. In addition, the potential of biomarkers to facilitate the de, Validation of Cell-Based Assays in the GLP Setting: A Practical Guide to your collection on WonderClub |