Stem Cell Culture: Methods in Cell Biology Book

Contributors     xi
Preface     xv
Biography     xix
Derivation of Human Embryonic Stem Cells in Standard and Chemically Defined Conditions   Eric Chiao   Muriel Kmet   Barry Behr   Julie Baker
Introduction     1
Methods     2
Discussion     12
Materials     12
References     13
Autogeneic Feeders for the Culture of Undifferentiated Human Embryonic Stem Cells in Feeder and Feeder-Free Conditions   Andre Choo   Ang Sheu Ngo   Vanessa Ding   Steve Oh   Lim Sai Kiang
Introduction     16
Methods and Materials     17
Results     21
Discussion and Summary     25
References     28
Microporous Membrane Growth Substrates for Embryonic Stem Cell Culture and Differentiation   Steven D. Sheridan   Sonia Gil   Matthew Wilgo   Aldo Pitt
Introduction     30
Rationale     32
Methods     32
Summary     54
References     55
Culture of Rodent Spermatogonial Stem Cells, Male Germline Stem Cells of the Postnatal Animal   Hiroshi Kubota   Ralph L. Brinster
Introduction     60
Rationale     63
Methods     67
Materials     78
Discussion     80
References     81
Characterization of Human Amniotic Fluid Stem Cells and Their Pluripotential Capability   Laura Perin   Sargis Sedrakyan   Stafano Da Sacco   Roger De Filippo
Introduction     86
Amniotic Fluid Cell Composition and Stem Cells     87
In Vitro Potential of Amniotic Fluid Stem Cells     91
Ex Vivo and In Vivo Application of Amniotic Fluid Stem Cells     94
Discussion     97
References     98
Method to Isolate Mesenchymal-Like Cells from Wharton's Jelly of Umbilical Cord   Kiran Seshareddy   Deryl Troyer   Mark L. Weiss
Introduction     102
Rationale     103
Methods     103
Materials     110
UCMSCs and Culture Characteristics     115
Discussion     116
References     118
Isolation, Characterization, and Differentiation of Human Umbilical Cord Perivascular Cells (HUCPVCs)   J. Ennis   R. Sarugaser   A. Gomez   Dolores Baksh   John E. Davies
Introduction     122
Methods      123
Concluding Remarks     133
References     134
Hepatic Stem Cells and Hepatoblasts: Identification, Isolation, and Ex Vivo Maintenance   Eliane Wauthier   Eva Schmelzer   William Turner   Lili Zhang   Ed LeCluyse   Joseph Ruiz   Rachael Turner   M. E. Furth   Hiroshi Kubota   Oswaldo Lozoya   Claire Barbier   Randall McClelland   Hsin-lei Yao   Nicholas Moss   Andrew Bruce   John Ludlow   L. M. Reid
Introduction     139
Epithelial-Mesenchymal Relationship     139
Stem Cells and Lineage Biology     140
Liver Processing     150
Fractionation of Liver Cell Subpopulations     162
Feeder Cells     170
Extracellular Matrix     174
Culture Media     176
Monolayer Cultures of Cells     180
Three-Dimensional Systems     185
Nomenclature, Glossary, and Abbreviations     195
Details on the Preparation of Buffers/Reagents     207
Commercial Sources of Reagents     211
Sources of Primary and Secondary Antibodies for Immunoselection and for Characterization of the Cells     212
References      213
Culture of Pluripotent Neural Epithelial Progenitor Cells from E9 Rat Embryo   Ronghao Li   Jennie P. Mather
Introduction     228
Rationale     229
Methods     229
Materials     237
Discussion     237
Summary     238
References     239
Primary and Multipassage Culture of Human Fetal Kidney Epithelial Progenitor Cells   Deryk Loo   Claude Beltejar   Jeff Hooley   Xiaolin Xu
Introduction     242
Rationale     243
Methods     245
Materials     250
Discussion     253
Summary     254
References     254
Isolation of Human Mesenchymal Stem Cells from Bone and Adipose Tissue   Susan H. Bernacki   Michelle E. Wall   Elizabeth G. Loboa
Introduction     258
Isolation of hMSCs from Trabecular Bone     259
Isolation of hMSCs from Adipose Tissue     263
Growth, Subculture, and Cryopreservation of Undifferentiated hMSCs     265
Surface Marker Characterization of hMSCs     267
hMSC Differentiation Assays for Osteogenic, Adipogenic, and Chondrogenic Pathways     270
Summary      276
References     276
Culture of Mesenchymal Stem/Progenitor Cells in Adhesion-Independent Conditions   Dolores Baksh   John E. Davies
Introduction     280
Suspension Culture of Bone Marrow-Derived MPCs     280
Analysis of Suspension Culture     283
Mechanism of MPC Expansion in Suspension     288
Discussion     291
References     292
Purification and Long-Term Culture of Multipotent Progenitor Cells Affiliated with the Walls of Human Blood Vessels: Myoendothelial Cells and Pericytes   Mihaela Crisan   Bridget Deasy   Manuela Gavina   Bo Zheng   Johnny Huard   Lorenza Lazzari   Bruno Peault
Introduction     297
Materials and Methods: Cell Analysis and Purification by Flow Cytometry     298
Materials and Methods: Long-Term Culture of Human Blood Vessel-Associated Progenitor Cells     301
Materials and Methods: Proliferation Kinetics of Human Myoendothelial Cells and Pericytes     303
Materials and Methods: Genotypic and Phenotypic Analyses of Long-Term Cultured Cells     304
Conclusion     306
References     308
Isolation and Culture of Colon Cancer Stem Cells   Patrizia Cammareri   Ylenia Lombardo   Maria Giovanna Francipane   Sebastino Bonventre   Matilde Todaro   Giorgio Stassi
Introduction     312
Identification of CSCs in Colon Cancer Tissue     314
Isolation and Propagation of Colon CSCs     315
Discussion     322
References     323
Isolation and Establishment of Human Tumor Stem Cells   Penelope E. Roberts
Introduction     326
Rationale     327
Methods     327
Results     336
Discussion     338
Materials     340
References     341
Stem Cells from Cartilaginous and Bony Fish   David W. Barnes   Angela Parton   Mitsuru Tomana Jae-Ho Hwang   Anne Czechanski   Lanchun Fan   Paul Collodi
Introduction to Models and Uses     344
Embryonal Stem Cells     348
Tissue-Specific Stem Cells     353
Outlook and Future Contributions     361
References     362
Index     369
Volumes in Series     379