Septic Shock Methods and Protocols Book

Part I. Endotoxin. Assay of Endotoxin by Limulus Amebocyte Lysate, Paul A. Ketchum and Thomas J. Novitsky. Preparation of Endotoxin from Pathogenic Gram-Negative Bacteria, Alexander Shnyra, Michael Luchi, and David C. Morrison. Assay of Anti-Endotoxin Antibodies, Lore Brade. Isolation of the Bactericidal/Permeability-Increasing Protein from Polymorphonuclear Leukocytes by Reversible Binding to Target Bacteria, Jerrold Weiss. Purification of Lipopolysaccharide Binding Protein, Didier Heumann. Part II. Other Bacterial Products. Purification of Strepoccal Pyrogenic Exotoxin A, Manuela Roggiani and Patrick M. Schlievert. Assay for Superantigens, Shiranee Sriskandan. Part III. Cytokines. Bioassay for Tumor Necrosis Factors-a and b, Thomas J. Evans. Assay of Soluble Tumor Necrosis Factor Receptors, Maarten G. Bouma and Wim A. Buurman. Whole-Blood Assays for Cytokine Production, Daniel G. Remick, David E. Newcomb, and Jon S. Friedland. Part IV. Nitric Oxide and Other Reactive Nitrogen Intermediates. Nitric Oxide Synthase Inhibitors, Dilani K. Siriwardena, Hajime Tagori, Christoph Thiemermann. In Situ Detection of Nitric Oxide, Tadeusz Malinski. Immunochemical Detection of Nitric Oxide Synthase in Human Tissue, Lee D. K. Buttery and Julia M. Polak. Anti-Nitrotyrosine Antibodies for Immunohishemistry, Liliana Viera, Yao Zu Ye, and Joseph S. Beckman. Detection of Peroxynitrite in Biological Fluids, Stuart Malcolm, Raymond Foust III, Caryn Hertkorn, and Harry Ischiropoulos. Part V. Cell Culture Techniques. Myocardial Cells in Culture to Study Effects of Cytokines, Kevin A. Krown and Roger A. Sabbadini. Culture of Primary Human Bronchial Epithelial Cells, Joanna Picot. Primary Culture of Human Proximal Renal Tubular Epithelial Cells, Paul A. Glynne. Index