Recombinant DNA and Biotechnology : A Guide for Students Book

	Preface
	Laboratory Biosafety
	Aseptic Technique
1	An Overview of Biotechnology	3
2	Genes, Genetics, and Geneticists	33
3	An Overview of Molecular Biology	53
4	Applying Molecular Biology: Recombinant DNA Technology	91
5	Constructing a Paper Helix	118
6	DNA Polymerase: the Replicator	120
7	From Genes to Proteins	122
	Does Antisense Make Sense?	125
8	Sizes of the Escherichia coli and Human Genomes	127
9	Extraction of Bacterial DNA	130
10	DNA Scissors	134
11	DNA Goes to the Races	136
12	Gel Electrophoresis of Precut Lambda DNA	140
13	Restriction Analysis of Lambda DNA	143
14	Recombinant Paper Plasmids	146
15	Restriction Analysis Challenge	149
16	Detection of Specific DNA Sequences: Fishing for DNA	152
	Hybridization Analysis	154
17	DNA Sequencing the Terminators	157
	Reading: Chain Terminators as Antiviral Drugs	160
18	The Polymerase Chain Reaction	162
19	Transformation of Escherichia coli	168
20	Conjugative Transfer of Antibiotic Resistance in Escherichia coli	172
21	Transduction of an Antibiotic Resistance Gene	175
22	Agrobacterium tumefaciens: Nature's Plant Genetic Engineer	179
	Reading: Methods of Plant Genetic Engineering	181
23	Generating Genetic Variation: the Meiosis Game	184
24	Analyzing Genetic Variation: DNA Typing	190
25	A Mix-Up at the Hospital	199
26	A Paternity Case	201
27	The Case of the Bloody Knife	203
28	The Molecular Basis of Genetic Diseases	205
	Reading: The Molecular Genetics of Cancer	209
29	Science, Technology, and Society	217
30	Weighing Technology's Risks and Benefits	220
31	Debating the Risks of Biotechnology	226
	Reading: Environmental Introductions of Genetically Engineered Organisms	230
32	A Decision-Making Model for Bioethical Issues	235
33	Bioethics Case Study Gene Therapy	240
34	Bioethics Case Study Genetic Screening	245
35	Careers in Biotechnology	248
	Appendix A: Templates	255
	Glossary	335
	Index	343