Practical protein crystallography Book

	Foreword to the Second Edition
	Preface to the Second Edition
	Acknowledgments
1	Laboratory Techniques
1.1	Preparing Protein Samples	1
1.2	Protein Crystal Growth	6
1.3	Crystal Storage and Handling	17
1.4	Crystal Soaking	18
1.5	Anaerobic Crystals	20
2	Data Collection Techniques
2.1	Preparing Crystals for Data Collection	23
2.2	Optical Alignment	31
2.3	X-Ray Sources	34
2.4	Preliminary Characterization	39
2.5	Heavy-Atom Derivative Scanning with Film	64
2.6	Overall Data Collection Strategy	67
2.7	Overview of Older Film Techniques	73
2.8	Four-Circle Diffractometer Data Collection	74
2.9	Area Detector Data Collection	76
2.10	Image Plate Data Collection	82
2.11	Synchrotron Radiation Light Sources	84
2.12	Data Reduction	87
3	Computational Techniques
3.1	Terminology	93
3.2	Basic Computer Techniques	102
3.3	Data Reduction and Statistical Analysis	115
3.4	The Patterson Synthesis	124
3.5	Fourier Techniques	137
3.6	Isomorphous Replacement Phasing	145
3.7	Molecular Replacement	162
3.8	Noncrystallographic Symmetry	171
3.9	Density Modification	176
3.10	Multiwavelength Data with Anomalous Scattering	182
3.11	Refinement of Coordinates	193
3.12	Fitting of Maps	217
3.13	Analysis of Coordinates	265
4	XtalView Tutorials
4.1	Installation	271
4.2	Obtaining Help	272
4.3	XView	272
4.4	A Typical Manual Fitting Session with Xfit	303
4.5	Interfacing to Other Programs	326
5	Protein Crystallography Cookbook
5.1	Multiple Isomorphous Replacement	329
5.2	Mutant Studies	366
5.3	Substrate - Analog Example	380
5.4	Molecular Replacement	384
5.5	Multiwavelength Anomalous Dispersion (MAD) Phasing	388
6	Cryocrystallography: Basic Theory and Methods
6.1	Overview	410
6.2	Theory	411
6.3	Room Temperature versus Low-Temperature Crystallography	413
6.4	Cryogenic Safety	415
6.5	Equipment	416
6.6	Crystal Loop Mounting Techniques	423
6.7	Crystal Storage Overview	427
6.8	Crystal Removal and Storage	432
6.9	Crystal Handling	441
App. A	Crystallographic Equations in Computer Code	445
App. B	Useful Web Sites	455
	Index	463