Practical Molecular Virology, Vol. 8 Book

The Retroviral Life Cycle and the Molecular Construction of Retrovirus Vectors. Construction of Retroviral Packaging Cell Lines. Making High Titer Retroviral Producer Cells. Methods of Titrating Nonselectable Recombinant Retroviruses. Selectable Markers for Eukaryotic Cells. Virus Genome Detection by the Polymerase Chain Reaction (PCR). Synctial Assays. Neutralization of Virus. Vesicular Stomatitis Virus Pseudotypes of Retroviruses. Cell Lineage Studies Using Retroviruses. Retroviral Vectors as Insertional Mutagents. Assessment of Compounds for Anti-HIV Activity. Analysis of Protein Factors that Interact with Adenovirus Early Promoters. COS Cell Expression. Papillomaviruses and Assays for Transforming Genes. Herpes Simplex Virus Life Cycle and the Design of Viral Vectors. Growth of Herpes Simplex Virus and Purification of Viral DNA. Vectors Based on Epstein-Barr Virus. Immortalization of Human B-Lymphocytes by Epstein-Barr Virus. Vaccinia Virus as an Expression Vector. Isolation and Handling of Recombinant Vaccinia Viruses. Design and Construction of Poliovirus Epitope Expression Vectors. Growth and Characterization of Poliovirus Antigen Chimeras. Expression Vectors for the Construction of Hybrid Ty-VLPs. Production and Purification of Hybrid Ty-VLPs. Baculovirus Expression Vectors: Choice of Expression Vector. Baculovirus Expression Vectors: Generating a Recombinant Baculovirus. Baculovirus Expression Vectors: Scale-Up and Downstream Processing. Index.