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Plant Cell, Tissue and Organ Culture: Fundamental Methods Book

Plant Cell, Tissue and Organ Culture: Fundamental Methods
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Plant Cell, Tissue and Organ Culture: Fundamental Methods, This manual provides all relevant protocols for basic and applied plant cell and molecular technologies, such as histology, electron microscopy, cytology, virus diagnosis, gene transfer and PCR. Also included are chapters on laboratory facilities, operati, Plant Cell, Tissue and Organ Culture: Fundamental Methods
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  • Plant Cell, Tissue and Organ Culture: Fundamental Methods
  • Written by author O. L. Gamborg, G. C. Phillps
  • Published by Springer-Verlag New York, LLC, 9/19/1995
  • This manual provides all relevant protocols for basic and applied plant cell and molecular technologies, such as histology, electron microscopy, cytology, virus diagnosis, gene transfer and PCR. Also included are chapters on laboratory facilities, operati
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Book Categories

Authors

Ch. 1 Laboratory Facilities, Operation, and Management 3
Ch. 2 Media Preparation and Handling 21
Ch. 3 Sterile Techniques 35
Ch. 4 Micropropagation by Proliferation of Axillary Buds 45
Ch. 5 Adventitious Shoot Proliferation 55
Ch. 6 Plant Regeneration by Organogenesis from Callus and Cell Suspension Cultures 67
Ch. 7 Plant Regeneration from Callus and Cell Suspension Cultures by Somatic Embryogenesis 81
Ch. 8 Direct Somatic Embryogenesis 91
Ch. 9 Conifer Somatic Embryogenesis, Embryo Development, Maturation Drying and Plant Formation 103
Ch. 10 Meristem Culture for Virus Elimination 117
Ch. 11 Embryo Culture and Embryo Rescue for Wide Cross Hybrids 129
Ch. 12 Anther Culture for Doubled Haploid Production 143
Ch. 13 Microspore Culture for Haploid Plant Production 155
Ch. 14 Protoplast Isolation and Culture 167
Ch. 15 Agrobacterium-Mediated Transformation 181
Ch. 16 Direct DNA Transfer into Intact Plant Cells Via Microprojectile Bombardment 197
Ch. 17 Botanical Microtechnique for Plant Cultures 217
Ch. 18 Transmission and Scanning Electron Microscopy for Plant Protoplasts, Cultured Cells, and Tissues 229
Ch. 19 Indirect Immunofluorescence Techniques for Cultured Plant Cells and Protoplasts 239
Ch. 20 Staining Procedures for Chromosome Analysis 251
Ch. 21 Enzyme-Linked Immunosorbent Assay (ELISA) Methods to Certify Pathogen (Virus)-Free Plants 267
Ch. 22 Applications of Polymerase Chain Reaction (PCR) to Plant Genome Analysis 281
Appendix A: Basal Media for Plant Cell and Tissue Culture 301
Appendix B: Lists of Compounds and Molecular Weights 309
Appendix C: Answers to Study Questions (Compiled by the authors and the editors) 315
Appendix D: List of Suppliers (Compiled by the authors and the editors) 327
Appendix E: Glossary of Terms used in Plant Cellular and Developmental Biology 337
Subject Index 349


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Plant Cell, Tissue and Organ Culture: Fundamental Methods, This manual provides all relevant protocols for basic and applied plant cell and molecular technologies, such as histology, electron microscopy, cytology, virus diagnosis, gene transfer and PCR. Also included are chapters on laboratory facilities, operati, Plant Cell, Tissue and Organ Culture: Fundamental Methods

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Plant Cell, Tissue and Organ Culture: Fundamental Methods, This manual provides all relevant protocols for basic and applied plant cell and molecular technologies, such as histology, electron microscopy, cytology, virus diagnosis, gene transfer and PCR. Also included are chapters on laboratory facilities, operati, Plant Cell, Tissue and Organ Culture: Fundamental Methods

Plant Cell, Tissue and Organ Culture: Fundamental Methods

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Plant Cell, Tissue and Organ Culture: Fundamental Methods, This manual provides all relevant protocols for basic and applied plant cell and molecular technologies, such as histology, electron microscopy, cytology, virus diagnosis, gene transfer and PCR. Also included are chapters on laboratory facilities, operati, Plant Cell, Tissue and Organ Culture: Fundamental Methods

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