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Ch. 1 | Laboratory Facilities, Operation, and Management | 3 |
Ch. 2 | Media Preparation and Handling | 21 |
Ch. 3 | Sterile Techniques | 35 |
Ch. 4 | Micropropagation by Proliferation of Axillary Buds | 45 |
Ch. 5 | Adventitious Shoot Proliferation | 55 |
Ch. 6 | Plant Regeneration by Organogenesis from Callus and Cell Suspension Cultures | 67 |
Ch. 7 | Plant Regeneration from Callus and Cell Suspension Cultures by Somatic Embryogenesis | 81 |
Ch. 8 | Direct Somatic Embryogenesis | 91 |
Ch. 9 | Conifer Somatic Embryogenesis, Embryo Development, Maturation Drying and Plant Formation | 103 |
Ch. 10 | Meristem Culture for Virus Elimination | 117 |
Ch. 11 | Embryo Culture and Embryo Rescue for Wide Cross Hybrids | 129 |
Ch. 12 | Anther Culture for Doubled Haploid Production | 143 |
Ch. 13 | Microspore Culture for Haploid Plant Production | 155 |
Ch. 14 | Protoplast Isolation and Culture | 167 |
Ch. 15 | Agrobacterium-Mediated Transformation | 181 |
Ch. 16 | Direct DNA Transfer into Intact Plant Cells Via Microprojectile Bombardment | 197 |
Ch. 17 | Botanical Microtechnique for Plant Cultures | 217 |
Ch. 18 | Transmission and Scanning Electron Microscopy for Plant Protoplasts, Cultured Cells, and Tissues | 229 |
Ch. 19 | Indirect Immunofluorescence Techniques for Cultured Plant Cells and Protoplasts | 239 |
Ch. 20 | Staining Procedures for Chromosome Analysis | 251 |
Ch. 21 | Enzyme-Linked Immunosorbent Assay (ELISA) Methods to Certify Pathogen (Virus)-Free Plants | 267 |
Ch. 22 | Applications of Polymerase Chain Reaction (PCR) to Plant Genome Analysis | 281 |
Appendix A: Basal Media for Plant Cell and Tissue Culture | 301 | |
Appendix B: Lists of Compounds and Molecular Weights | 309 | |
Appendix C: Answers to Study Questions (Compiled by the authors and the editors) | 315 | |
Appendix D: List of Suppliers (Compiled by the authors and the editors) | 327 | |
Appendix E: Glossary of Terms used in Plant Cellular and Developmental Biology | 337 | |
Subject Index | 349 |
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Add Plant Cell, Tissue and Organ Culture: Fundamental Methods, This manual provides all relevant protocols for basic and applied plant cell and molecular technologies, such as histology, electron microscopy, cytology, virus diagnosis, gene transfer and PCR. Also included are chapters on laboratory facilities, operati, Plant Cell, Tissue and Organ Culture: Fundamental Methods to the inventory that you are selling on WonderClubX
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Add Plant Cell, Tissue and Organ Culture: Fundamental Methods, This manual provides all relevant protocols for basic and applied plant cell and molecular technologies, such as histology, electron microscopy, cytology, virus diagnosis, gene transfer and PCR. Also included are chapters on laboratory facilities, operati, Plant Cell, Tissue and Organ Culture: Fundamental Methods to your collection on WonderClub |