PCR Technology: Principles and Applications for DNA Amplification Book

PART I: BASIC METHODOLOGY

1. The Design and Optimization of the PCR, Randall K. Saiki

2. Taq DNA Polymerase, David Gelfand

3. PCR Automatin, Christian Oste

4. Simple and Rapid Preparation of Samples for PCR, Russell Higuchi

PART II: RESEARCH APPLICATIONS

5. Direct Sequencing of In Vitro Amplified DNA, Ulf Gyllensten

6. Using PCR to Engineer DNA, Russell Higuchi

7. Mutation Detection by PCR, GC-Clamps, and Denaturing Gradient Gel Electrophoresis, Richard M. Myers, Val C. Sheffield, and David R. Cox

8. Detection of Gene Expression, Ernest S. Kawasaki and Alice M. Wang

9. PCR Amplification of Specific Sequences from cDNA Library, J.-S. Tung, B.L. Daugherty, L. O'Neill, S.W. Law, J. Han, and G.E. Mark

10. Inverse Polymerase Chain Reaction, Howard Ochman, James W. Ajioka, Dan Garza, and Daniel L. Hartl

11. Alu PCR: The Use of Repeat Sequence Primers for Amplification of Human DNA from Complex Sources, David L. Nelson and C. Thomas Caskey

12. A New Approach to Constructing Genetic Maps: PCR Analysis of DNA Sequences in Individual Gametes, Norman Arnheim

13. Evolutionary Analysis via PCR, Thomas D. Kocher and Thomas H. White

PART III: MEDICAL APPLICATIONS

14. Use of PCR in the Diagnosis of Monogenic Disease, Haig H. Kazazian, Jr.

15. Diagnosis of New Mutation Diseases Using the Polymerase Chain Reaction, R.A. Gibbs, J.S. Chamberlain, and C. Thomas Caskey

16. HLA Class II Gene Polymorphism: DNA Typing, Evolution, and Relationship to Disease Susceptibility, Henry A. Erlich and Teodorica L. Bugawan

17. Applications of PCR to theAnalysis of Biological Evidence, Cecilia H. von Beroldingen, Edward T. Blake, Russell Higuchi, George F. Sensabaugh, and Henry A. Erlich

18. Detection of ras Oncogenes Using PCR, Johannes L. Bos

19. Application of PCR to the Detection of Human Infectious Diseases, Shirley Kwok and John J. Sninsky