Nonisotopic DNA Probe Techniques Book

	Contributors
	Preface
Pt. 1	Introduction
1	Nucleic Acid Hybridization Test Formats: Strategies and Applications
I	Introduction	3
II	Nucleic Acid Labels	4
III	Nucleic Acid Labeling Procedures	6
IV	Detection of Labels and Nucleic Acid Hybridization Assay Sensitivity	9
V	Patents	16
VI	Conclusions	l9
	References	19
2	Nonradioactive Labeling Methods for Nucleic Acids
I	Overview	30
II	Methods for Enzymatic Labeling	37
III	Methods for Chemical Labeling	49
IV	Methods for Chemical Labeling of DNA, RNA, and Oligodeoxynucleotides with Marker Enzymes	62
V	Overview of Factors Influencing Hybridization	66
VI	Overview of Detection Systems	69
	References	78
Pt. 2	Detection Methods
3	Detection of Alkaline Phosphatase by Time-Resolved Fluorescence
I	Introduction	95
II	Materials	97
III	Procedures	102
	References	110
4	Detection of Alkaline Phosphatase by Bioluminescence
I	Introduction	113
II	Materials	114
III	Procedures	117
IV	Conclusions	123
	References	124
5	Detection of DNA on Membranes with Alkaline Phosphatase-Labeled Probes and Chemiluminescent AMPPD Substrate
I	Introduction	128
II	General Southern Blotting Procedure with Chemiluminescence	129
III	Two-Step Hybridization Southern Blotting Procedure--Detection of Single-Copy Genes	137
IV	Conclusions	144
	References	144
6	Detection of Alkaline Phosphatase by Colorimetry
I	Introduction	147
II	Labeling and Detection Strategies	148
III	Hybridization of Biotinylated Probes	151
IV	Detection of Biotinylated Probes	153
V	In Situ Hybridization	155
VI	Conclusions	163
	References	164
7	Detection of Horseradish Peroxidase by Enhanced Chemiluminescence
I	Introduction	167
II	Materials	173
III	Procedures	175
	References	182
8	Detection of Horseradish Peroxidase by Colorimetry
I	Introduction	185
II	Materials	191
III	Procedures	191
IV	Conclusions	198
	References	199
9	Detection of Glucose 6-Phosphate Dehydrogenase by Bioluminescence
I	Introduction	203
II	Materials	205
III	Procedures	212
IV	Conclusions	223
	References	224
10	Detection of Lanthanide Chelates by Time-Resolved Fluorescence
I	Introduction	228
II	Indirect Labeling	229
III	Chemical Europium Labeling of DNA Probes	235
IV	Enzymatic Europium Labeling of DNA Probes	244
V	Europium-Labeled Oligonucleotides	246
	References	260
11	Detection of Lanthanide Chelates and Multiple Labeling Strategies Based on Time-Resolved Fluorescence
I	Introduction	263
II	Materials	265
III	Procedures	268
	References	273
12	Detection of Acridinium Esters by Chemiluminescence
I	Introduction	276
II	Materials	287
III	Procedures	290
	References	308
13	Detection of Energy Transfer and Fluorescence Quenching
I	Introduction	312
II	Materials	327
III	Procedures	339
	References	351
	Index	353