Molecular Embryology: Methods and Protocols, Vol. 461 Book

	Preface
	List of Color Plates
	Contributors
Pt. I	The Mouse Embryo	1
1	The Mouse as a Developmental Model	3
2	Culture of Postimplantation Mouse Embryos	7
3	Organ Culture in the Analysis of Tissue Interactions	23
4	Treatment of Mice with Retinoids In Vivo and In Vitro: Skeletal Staining	33
5	Cell Grafting and Labeling in Postimplantation Mouse Embryos	41
6	Production of Transgenic Rodents by the Microinjection of Cloned DNA into Fertilized One-Celled Eggs	61
7	Cre Recombinase Mediated Alterations of the Mouse Genome Using Embryonic Stem Cells	101
8	Gene Trapping in Mouse Embryonic Stem Cells	123
9	Production and Growth of Conditionally Immortal Cell Lines from the H-2K[superscript b]tsA58 Transgenic Mouse	139
10	Reporter Genes for the Study of Transcriptional Regulation in Transgenic Mouse Embryos	159
11	Application of IacZ Transgenic Mice to Cell Lineage Studies	183
12	Mouse Primordial Germ Cells: Isolation and In Vitro Culture	201
Pt. II	Chicken Embryo	213
13	The Avian Embryo: An Overview	215
14	Chick Embryos: Incubation and Isolation	221
15	New Culture	225
16	Grafting Hensen's Node	245
17	Grafting of Somites	255
18	Notochord Grafts	265
19	Transplantation of Avian Neural Tissue	273
20	Grafting of Apical Ridge and Polarizing Region	281
21	Tissue Recombinations in Collagen Gels	293
22	Quail-Chick Chimeras	305
23	Using Fluorescent Dyes for Fate Mapping, Lineage Analysis, and Axon Tracing in the Chick Embryo	319
Pt. III	Amphibian Embryo	329
24	An Overview of Xenopus Development	331
25	Mesoderm Induction Assays	341
26	Experimental Embryological Methods for Analysis of Neural Induction in the Amphibian	351
27	A Method for Generating Transgenic Frog Embryos	393
28	Axolotl/newt	415
Pt. IV	Zebrafish	429
29	The Zebrafish: An Overview of Its Early Development	431
30	Small-Scale Marker-Based Screening for Mutations in Zebrafish Development	441
31	Transgenic Zebrafish	461
32	Microinjection of DNA, RNA, and Protein into the Fertilized Zebrafish Egg for Analysis of Gene Function	487
33	Retinoids in Nonmammalian Embryos	491
Pt. V	Nonvertebrate Chordates	511
34	Protochordates	513
Pt. VI	Retroviruses	517
35	Gene Transfer to the Rodent Embryo by Retroviral Vectors	519
36	Gene Transfer in Avian Embryos Using Replication-Competent Retroviruses	539
Pt. VII	Molecular Techniques	553
37	Subtractive Hybridization and Construction of cDNA Libraries	555
38	Differential Display of Eukaryotic mRNA	575
39	RT-PCR on Embryos Using Degenerate Oligonucleotide Primers	591
40	Single-Cell RT-PCR cDNA Subtraction	601
41	In Situ Hybridization of Radioactive Riboprobes to RNA in Tissue Sections	611
42	In Situ Hybridization to RNA in Whole Embryos	623
43	Wholemount In Situ Hybridization to Xenopus Embryos	635
44	Whole-Mount In Situ Hybridization to Amphioxus Embryos	641
45	In Situ Hybridization to Sections (Nonradioactive)	645
46	Immunohistochemistry Using Polyester Wax	655
47	Immunohistochemistry on Whole Embryos	663
48	Whole Embryo Assays for Programmed Cell Death	667
49	Gene Interference Using Antisense Oligodeoxynucleotides on Whole Chick Embryos: Optimal Ring and Roller-Bottle Culture Technique	673
50	Protein Techniques: Immunoprecipitation, In Vitro Kinase Assays, and Western Blotting	699
Pt. VIII	Microscopy and Photography	709
51	Microscopy and Photomicrography Techniques	711
	Index	735