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Contributors ix
Preface xi
1 Overview of the Various Types of Mass Spectrometers that are Used in Drug Discovery and Drug Development 1
Gérard Hopfgartner
2 Utility of High-Resolution Mass Spectrometry for New Drug Discovery Applications 37
William Bart Emary and Nanyan Rena Zhang
3 Quantitative Mass Spectrometry Considerations in a Regulated Environment 55
Mohammed Jemal and Yuan-Qing Xia
4 Mass Spectrometry for Quantitative In Vitro ADME Assays 97
Jun Zhang and Wilson Z. Shou
5 Metabolite Identifi cation Using Mass Spectrometry in Drug Development 115
Natalia Penner, Joanna Zgoda-Pols, and Chandra Prakash
6 MS Analysis of Biological Drugs, Proteins, and Peptides 149
Yi Du, John Mehl, and Pavlo Pristatsky
7 Characterization of Impurities and Degradation Products in Small Molecule Pharmaceuticals and Biologics 191
Hui Wei, Guodong Chen, and Adrienne A. Tymiak
8 Liquid Extraction Surface Analysis (LESA): A New Mass Spectrometry-Based Technique for Ambient Surface Profiling 221
Daniel Eikel and Jack D. Henion
9 MS Applications in Support of Medicinal Chemistry Sciences 239
Maarten Honing, Benno Ingelse, and Birendra N. Pramanik
10 Imaging Mass Spectrometry of Proteins and Peptides 277
Michelle L. Reyzer and Richard M. Caprioli
11 Imaging Mass Spectrometry for Drugs and Metabolites 303
Stacey R. Oppenheimer
12 Screening Reactive Metabolites: Role of Liquid Chromatography–High-Resolution Mass Spectrometry in Combination with “Intelligent” Data Mining Tools 339
Shuguang Ma and Swapan K. Chowdhury
13 Mass Spectrometry of siRNA 357
Mark T. Cancilla and W. Michael Flanagan
14 Mass Spectrometry for Metabolomics 387
Petia Shipkova and Michael D. Reily
15 Quantitative Analysis of Peptides with Mass Spectrometry: Selected Reaction Monitoring or High-Resolution Full Scan? 403
Lieve Dillen and Filip Cuyckens
Index 427
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