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| Preface | iii | |
| 1 | Introduction | 1 |
| 1.1 | Terminology | 4 |
| 1.2 | Basic Concepts of Column Chromatography | 8 |
| 1.3 | Brief History of Ion-Exchange Chromatography | 15 |
| 1.4 | Comparison of Ion-Exchange Chromatography with Other Chromatographic Techniques | 20 |
| 1.5 | Characteristics of Ion-Exchange Chromatography of Proteins | 27 |
| 1.6 | Various Elution Procedures of Ion-Exchange Chromatography of Proteins | 30 |
| 2 | Theoretical Aspects | 35 |
| 2.1 | General Theories of Chromatography | 36 |
| 2.2 | Theory of Ion-Exchange Chromatography of Proteins | 78 |
| 3 | Ion-Exchange Equilibria | 107 |
| 3.1 | Theoretical Treatment | 108 |
| 3.2 | Experimental Methods | 121 |
| 3.3 | Experimental Data | 125 |
| 4 | Diffusion in the Ion Exchanger | 145 |
| 4.1 | Theoretical Treatment | 145 |
| 4.2 | Experimental Methods | 151 |
| 4.3 | Experimental Data | 156 |
| 5 | Axial Dispersion in the Ion-Exchange Column | 161 |
| 6 | Experimental Methods and Apparatus | 171 |
| 6.1 | Apparatus | 173 |
| 6.2 | Pretreatment | 179 |
| 6.3 | Preparation of Ion-Exchange Gel Columns | 180 |
| 6.4 | Method for Sample Introduction | 183 |
| 6.5 | Method for Elution | 186 |
| 6.6 | Regeneration of the Column | 187 |
| 6.7 | Concentration of Fractions | 187 |
| 6.8 | Other Subjects | 189 |
| 7 | Ion Exchangers | 193 |
| 8 | Factors Affecting Separation Behavior | 209 |
| 8.1 | Elution Behavior in Linear Gradient Elution | 210 |
| 8.2 | Prediction of Peak Position and Peak Width in Linear Gradient Elution by Simplified Methods | 226 |
| 8.3 | Resolution in Linear Gradient Elution | 238 |
| 8.4 | Stepwise Elution | 265 |
| 8.5 | Other Special Separation Methods and Apparatus | 277 |
| 8.6 | Summary and Additional Comments | 293 |
| 9 | Large-Scale Operation | 305 |
| 9.1 | General Considerations | 306 |
| 9.2 | Applications | 317 |
| 9.3 | Problems Encountered in Actual Operation | 326 |
| 10 | Design Calculation Procedure | 329 |
| 10.1 | Linear Gradient Elution | 330 |
| 10.2 | Stepwise Elution | 341 |
| Concluding Remarks and Future Aspects | 347 | |
| Appendix | 349 | |
| A | Derivation of the Value of K[subscript S] | 349 |
| B | Derivation of Equation (2.59) | 351 |
| C | Analytical Solutions for a Change in Ionic Strength in Stepwise Elution and Linear Gradient Elution | 352 |
| D | Numerical Calculation Procedure | 354 |
| E | A Solution for the Peak Width in Linear Gradient Elution Under the Quasi-Steady State | 355 |
| F | Values of the Molecular Diffusion Coefficient and its Estimation Method | 359 |
| Contents | vii | |
| Symbols | 363 | |
| References | 377 | |
| Index | 395 |
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Add Ion Exchange Chromatography of Proteins, Showing chemists how to predict ion-exchange chromatography (IEC) separation behavior and how to determine appropriate operating conditions, this reference illustrates procedures, apparatus, and types of ion exchangers, emphasizing the design and applicat, Ion Exchange Chromatography of Proteins to the inventory that you are selling on WonderClubX
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Add Ion Exchange Chromatography of Proteins, Showing chemists how to predict ion-exchange chromatography (IEC) separation behavior and how to determine appropriate operating conditions, this reference illustrates procedures, apparatus, and types of ion exchangers, emphasizing the design and applicat, Ion Exchange Chromatography of Proteins to your collection on WonderClub |