Identification Methods in Applied and Environmental Microbiology Book

	Contributors
	Preface
	Identification of Medically Important Pseudomonas Species Using Computerized Methods	1
	Conventional Phenotypic Tests	2
	Commercial Identification Systems	7
	One-dimensional SDS-polyacrylamide Gel Electrophoresis	10
	Fatty-Acid Profiling	15
	Towards an Improved Classification of Pseudomonas	21
	DNA:rRNA Hybridizations	22
	Natural Relationships in Pseudomonas	27
	Dedication	39
	The Identification and Classification of Rhizobium and Bradyrhizobium	45
	Characteristics of the Genera	45
	Identification of the Root Nodule Bacteria	47
	The Species of Rhizobium	56
	Strain Differences within Species	57
	Views on Classification	59
	Gram-Negative Methylotrophic Rods and Cocci	67
	Isolation	67
	Obligate Methylotrophs	68
	Facultative Methylotrophs	77
	Isolation and Identification of Autotrophic Nitrifying Bacteria	87
	Isolation of Nitrifying Bacteria	88
	Classification and Identification	90
	Phenotypic Identification of Acetic Acid Bacteria	103
	Identification Tests	103
	Identification of Legionella Species	111
	Isolation	111
	Confirmation of Legionella Presumptives	114
	Subtyping of L. pueumophila	121
	Other Identification Schemes	123
	Identification and Typing of Enterobacteriaceae by Computerized Methods	127
	Conventional Phenotypic Tests	128
	Commercial Identification Systems	138
	One-dimensional SDA-polyacrylamide Gel Electrophoresis	145
	Identification and Biotyping of Campylobacters	151
	Materials and Methods	152
	Aeromonadaceae and Vibrionaceae	163
	Taxonomic Considerations	163
	Identification Techniques	169
	Identification of Chromobacterium, Janthinobacterium and Iodobacter Species	183
	Isolation and Maintenance	184
	Recognition of Pigmented and Unpigmented Members of the Genera	185
	Tests for Distinguishing between the Species	188
	Growth Temperatures	189
	Species Identification and Epidemiological Typing of the Staphylococci	193
	Identification of Staphylococci	193
	Subspecific Recognition Methods	201
	Typing for Epidemiology	215
	Streptococci and Related Organisms	221
	Isolation	221
	Test Methods	222
	Streptococci	225
	Animal Streptococci	234
	Lactococci	238
	Vagococci	240
	Enterococci	240
	Identification of Some Common Bacillus Thermophiles Using the API System and a Microcomputer	251
	Isolation	255
	Identification	255
	Pyrolysis Mass Spectrometry in the Identification of Bacillus Species	257
	Instrumentation	257
	Sample Preparation	258
	Pyrolysis and Mass Analysis	258
	Data Analysis	259
	Application of Data Analysis to Identification	261
	Identification Methods for Pathogenic Clostridium Species	263
	Culture Methods	266
	Isolation	269
	The Identification of Listeria Species	283
	Characteristics of the Genus	283
	Differentiation of the Species	287
	Protocol for the Routine Identification of Isolates	287
	Identification Methods	289
	Identification of Brochothrix by Intracellular and Surface Biochemical Composition	297
	Cellular Fatty Acids as a Means of Identification	299
	Identification of Pathogenic and Environmental Mycobacteria	311
	Methods for Mycobacterial Identification	313
	Identification and Typing of the Tuberculosis Complex	316
	Identification of Environmental Mycobacteria	319
	Additional Identification and Typing Methods	322
	Appendix: Media and Reagents	322
	Index	327