ICRF Handbook of Genome Analysis Book

Introduction.

Family Studies.

Complex Studies.

Constructing And Using Genetic Maps.

Automated Map Construction.

Polymorphic Markers.

DNA Typing.

Part II: Physical Mapping I: Cytogenetic Analysis.

Introduction.

Conventional Cytogenetics.

Solid Tumor Cytogenetics.

In Situ Hybridisation.

Chromosome Painting.

Chromosome Microdissection.

Chromosome Sorting And Analysis.

Digital Microscopy.

Part III: Physical Mapping II: Long-Range Mapping And Gene Isolation.

Introduction.

Somatic Cell Hybrid Approaches To Genome Mapping.

Long-Range Physical Mapping.

Integrating Genetic And Physical Maps.

Strategies For Rapid Isolation Of Genes From Genomic DNA:Cdna Techniques Screening Cdna Libraries By Transient Expression In Mammalian Cells.

Part IV: Sequencing And Sequence Analysis.

Introduction.

Sequencing Strategies.

Template Amplification And Purification.

Sequencing Chemistries.

Electrophoresis.

Sequence Labelling And Detection.

Automation.

Mutation Analysis.

Repeat Analysis.

Part V: Other Model Systems.

Introduction.

Mouse Mapping.

Somatic Gene Therapy.

Drosophila Melanogaster.

Caenorhabditis Elegan.

Saccharomyces Cerevisiae.

Escherichia Coli.

Problems In Plant Genome Analysis.

Arabidopsis Thaliana.

Part VI: Internet Resources.

Introduction.

Internet Resources And How To Use Them.

Unix Survival Kit.

Databases And Genome Resource Centers.

Appendices.

Materials, Media And Solutions.

Preparation Of Blood Bottles And Processing Of Blood Samples.

Commercial Suppliers.

Other Resources.

Useful Addresses And Internet Connections.

Basic Data On Human And Other Genomes.

Catalogue Of Mapped Human Disease Genes.

Mouse Gene Knock-Out Tables.

Chromosome Aberrations Associated With Cancer.

Glossary.

Index.