Flow Cytometry: First Principles Book

	Preface
	Acknowledgments (First Edition)
	Acknowledgments (Second Edition)
1	The Past as Prologue	1
2	Setting the Scene	11
3	Instrumentation: Into the Black Box	15
	Illumination of the Stream	16
	Centering Cells in the Illuminating Beam	18
	Detection of Signals from Cells	25
	Electronics	31
4	Information: Harnessing the Data	41
	Data Storage	41
	Data Analysis	43
5	Seeing the Light: Lasers, Fluorochromes, and Filters	59
	General Theory	59
	Lasers	61
	Fluorochromes	65
	Partitioning the Signal with Lenses, Filters, and Mirrors	72
	Spectral Compensation	76
6	Cells from Without: Leukocytes, Surface Proteins, and the Strategy of Gating	81
	Cells from Blood	82
	Staining for Surface Markers	87
	Controls	90
	Quantitation	95
	Sensitivity	98
	The Strategy of Gating	99
	Gating on Fluorescence	108
7	Cells from Within: Intracellular Proteins	115
	Methods for Permeabilizing Cells	116
	Examples of Intracellular Staining	118
8	Cells from Within: DNA in Life and Death	123
	Fluorochromes for DNA Analysis	123
	Ploidy	125
	Cell Cycle Analysis	131
	Two-Color Analysis for DNA and Another Parameter	142
	Chromosomes	147
	Apoptosis	150
	Necrosis	154
9	The Sorting of Cells	159
	Sorting Theory	159
	Characterization of Sorted Cells	166
	Alternative Methods for Sorting	170
	The Condition of Cells After Sorting	172
10	Disease and Diagnosis: The Clinical Laboratory	175
	The Hematology Laboratory	178
	The Pathology Laboratory	186
	Solid Organ Transplantation	189
	Comments	192
11	Out of the Mainstream: Research Frontiers	195
	Functional Assays	196
	The Aquatic Environment	202
	Reporter Molecules	207
	Microbiology	211
	Molecular Biology	213
	Multiplex Cytometry for Soluble Analytes	218
	Reproductive Technology	220
12	Flowing On: The Future	225
	General References	229
	Glossary	235
	Figure Credits	257
	Index	263