Experiments In Plant Tissue Culture Book

	List of tables
	Foreword
	Preface to the third edition
	Preface to the second edition
	Preface to the first edition
I	Background information
1	Culture of plant cells, tissues, and organs	3
2	Laboratory facilities	19
3	Aseptic techniques	25
4	Media composition and preparation	42
II	Experimental: Callus and callus-derived systems
5	Initiation and maintenance of callus	69
6	Organogenesis	82
7	Cell suspensions	92
8	Somatic embryogenesis	101
III	Experimental: Culture of organs and organized systems
9	Isolated roots	117
10	Micropropagation by bud proliferation	126
11	Anther and pollen cultures	136
IV	Experimental: Isolated cells
12	Transdifferentiation of parenchyma cells to tracheary elements	155
13	Isolation and culture of protoplasts	167
14	Protoplast fusion and somatic hybridization	183
V	Supplementary topics
15	Cryopreservation of germplasm	195
16	Production of secondary metabolites	204
17	Quantitation of procedures	214
	Abbreviations	229
	Glossary	231
	Commercial sources of supplies	235
	Formulations of tissue culture media	240
	Author index	243
	Subject index	248