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Book Categories |
| Preface to the first edition | ||
| Preface to the second edition | ||
| Protocols list | ||
| Abbreviations | ||
| 1 | Strategies for research in molecular biology | 1 |
| Strategies for gene isolation | 1 | |
| Studying gene structure and expression | 10 | |
| Interpretation of molecular biology experiments | 12 | |
| 2 | Construction of genomic libraries in [lambda] and cosmid vectors | 15 |
| Overview of library construction | 16 | |
| Representation | 17 | |
| The choice between [lambda] and cosmid libraries | 19 | |
| Vectors | 19 | |
| Library construction | 20 | |
| 3 | Construction of a cDNA library | 41 |
| Isolation of total RNA | 43 | |
| Preparation of poly(A[superscript +]) RNA | 44 | |
| cDNA synthesis | 46 | |
| Preparation of cDNA for cloning | 48 | |
| Establishment of the cDNA library | 55 | |
| Characterization of the cDNA library | 59 | |
| Amplification and storage of the cDNA library | 61 | |
| 4 | Nucleic acid labelling and detection | 63 |
| Radioactive labelling and detection | 69 | |
| Non-radioactive labelling and detection methods | 101 | |
| 5 | Immobilization of nucleic acids and hybridization analysis | 109 |
| Immobilization of nucleic acids on filters | 109 | |
| Hybridization analysis of immobilized nucleic acids | 133 | |
| Advanced hybridization techniques | 151 | |
| 6 | DNA sequencing | 157 |
| Preparation of template DNA | 158 | |
| The sequencing reactions | 166 | |
| Gel electrophoresis and autoradiography | 172 | |
| Building a long contiguous sequence | 179 | |
| Automated DNA sequencing | 183 | |
| DNA sequence storage and analysis | 184 | |
| 7 | The polymerase chain reaction | 187 |
| Methodology for PCR | 187 | |
| Cloning PCR products | 201 | |
| Sequencing PCR products | 203 | |
| Problems with PCR | 207 | |
| 8 | Protein expression in Escherichia coli | 209 |
| Factors affecting protein expression | 209 | |
| Protein fusion systems | 211 | |
| Expression of proteins in the periplasm | 217 | |
| Cofactor requirements | 219 | |
| Problems when scaling up expression | 219 | |
| 9 | Transcript mapping | 223 |
| Mapping by nuclease protection | 226 | |
| Primer-extension mapping | 239 | |
| A1 | Health hazards and safety procedures | 245 |
| A2 | Equipment for molecular biology research | 251 |
| A3 | Important Escherichia coli strains | 255 |
| A4 | Recipes and general procedures | 261 |
| A5 | Restriction endonucleases | 267 |
| A6 | DNA and RNA modification enzymes | 275 |
| A7: List of suppliers | 279 | |
| Index | 285 |
| Price | Condition | Delivery | Seller | Action |
| $99.99 | Digital |
| WonderClub |
Gregory Tanner
reviewed Essential Molecular Biology, Vol. 1 on January 22, 2009
Essential Molecular Biology
A very interesting book on genetics. In school biology was a fav class. This book also talks about eugenics, creating the perfect person. Enjoyed it very much.
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Add Essential Molecular Biology, Vol. 1, The two Essential Molecular Biology books in the Practical Approach Series are designed for the absolute beginner at gene cloning whether they be at the start of their career or an experienced researcher in another field. As with the first editions, Essential Molecular Biology, Vol. 1 to the inventory that you are selling on WonderClubX
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Add Essential Molecular Biology, Vol. 1, The two Essential Molecular Biology books in the Practical Approach Series are designed for the absolute beginner at gene cloning whether they be at the start of their career or an experienced researcher in another field. As with the first editions, Essential Molecular Biology, Vol. 1 to your collection on WonderClub |
