Electrophoresis in Practice Pt. A : Fundamentals and Proteins Book

A Fundamentals Electrophoresis: General/
Electrophoresis in non-restrictive gels/
Electrophoresis in restrictive gels;
Isotachophoresis;
Isoelectric focusing: Principles/
Gels for IEF/
Temperature/
Controlling the pH gradient/
The kinds of pH gradients/
Preparative isoelectric focusing/
Titration curve analysis;
Blotting: Principle/
Transfer methods/
Blotting membranes/
Buffers for electrophoretic transfers/
General staining/
Blocking/
Specific detection/
Protein sequencing/
Transfer problems;
Instrumentation: Current and voltage conditions/
Power supply/
Separation chambers/
Staining apparatus/
Automated electrophoresis/
Safety measures/
Environmental aspects;
Interpretation of electropherograms: Introduction/
Computer aided analysis;
Equipment: Instrumentation/
Laboratory equipment/
Consumables/
Chemicals

B Methods Method 1: PAGE of dyes;
Method 2: Agarose and immuno electrophoresis;
Method 3: Titration curve analysis;
Method 4: Native PAGE in amphoteric buffers;
Method 5: Agarose IEF;
Method 6: PAGIEF in rehydrated gels;
Method 7: SDS polyacrylamide electrophoresis;
Method 8: Semi-dry blotting of proteins;
Method 9: IEF in immobilized pH gradients;
Method 10: High-resolution 2D electrophoresis;
Method 11: Native PAGE of double stranded DNA fragments;
Method 12: Native PAGE of single stranded DNA fragments;
Method 13: Denaturing gradient gel electrophoresis;
Method 14: Denaturing PAGE of DNA fragments;
Method 15: Vertical PAGE

C Appendix Trouble-shooting guide: Isoelectric focusing/
PAGIEF with carrier ampholytes/
Agarose IEF with carrier ampholytes/
Immobilized pH gradients/
SDS electrophoresis/
Semi-dry blotting/
Two-dimensional electrophoresis (IPG-DALT)/
DNA electrophoresis/
Vertical electrophoresis

D References

E Index