Electron Microscopic Cytochemistry and Immunocytochemistry in Biomedicine Book

Pt. I	Methodology
Ch. 1	Electron Microscopic Cytochemistry of Enzymes
1.1	An Historical Perspective	3
1.2	Preservation of Enzymatic Activity and Ultrastructure
1.3	Basic Techniques for Major Groups of Enzymes
1.3.1	Oxidoreductases	51
1.3.2	Transferases	89
1.3.3	Hydrolases
1.3.4	Lyases - Carbonic Anhydrases	241
Ch. 2	Electron Microscopic Immunocytochemistry
2.1	An Historical Perspective	251
2.2	Preservation of Immunoreactivity and Ultrastructure	253
2.3	Major Visualization Techniques
2.3.1	Ferritin Techniques	275
2.3.2	Immunoelectron Microscopy by Enzyme-Labeled Antibodies	285
2.3.3	The Use of Avidin-Biotin Interaction in Immunocytochemistry and Immunoelectron Microscopy	293
2.3.4	Colloidal Gold Methods	303
2.3.5	Electron Microscopic Immunocytochemistry of Neuropeptides and Neurotransmitters	309
Ch. 3	Lectin Histochemistry and Cytochemistry	319
Ch. 4	In Situ Hybridization at the Electron Microscope Level	333
Ch. 5	Freeze-Fracture Cytochemistry and Fracture-Flip Cytochemistry	347
Ch. 6	Electron Microscopic Cytochemistry under the Scanning Electron Microscope	361
Ch. 7	Electron Microscopic Cytochemistry under the High-Voltage Electron Microscope	371
Ch. 8	Analytical Color Fluorescence Electron Microscopy	383
Pt. II	Major Contributions of Electron Microscopic Cytochemistry and Immunocytochemistry to Biomedicine
Ch. 9	Cell Organelles
9.1	Applications of Electron Microscopic Cytochemistry and Immunocytochemistry to the Study of the Plasma Membrane	399
9.2	Intercellular Junctions	417
9.3	Cytoskeleton	429
9.4	Contributions of Enzyme Cytochemistry and Immunocytochemistry to our Understanding of the Organization and Function of the Golgi Apparatus	441
9.5	Lysosomes	481
9.6	Peroxisomes	491
9.7	Mitochondria	505
Ch. 10	Endocrine Organs
10.1	Contributions of Ultrastructural Cytochemistry to our Understanding of Anterior Pituitary Cytophysiology	519
10.2	Adrenal Gland	529
10.3	Cytochemistry and Immunocytochemistry of the Thyroid Gland	585
Ch. 11	The Diffuse Endocrine System	597
Ch. 12	Cytochemistry and Immunocytochemistry in the Classification of Bone Marrow and Blood Cells and in the Diagnosis of Hematologic Disorders	611
Ch. 13	Muscle and Muscle Disease: Cytochemical and Immunocytochemical Aspects	639
Ch. 14	The Kidney and its Pathological Alterations	657
Ch. 15	Reproductive System
15.1	Immunocytochemistry of Secretory and Membrane Proteins of the Seminiferous Epithelium	673
15.2	Cytochemistry and Immunocytochemistry of the Female Reproductive System and Placenta	687
Ch. 16	Immunoelectron Microscopy of Normal and Abnormal Skin	697
Ch. 17	Cytochemical Studies on Photoreceptor Cells in the Retina	711
Ch. 18	Gingiva (Junctional Epithelium)	723
Ch. 19	Current Immunomorphologic Practices in Tumor Diagnosis: a Comprehensive Appraisal with Emphasis on Immunolabeling at the Light and Ultrastructural Levels	739
	Index	783