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DNA Isolation and Sequencing Book

DNA Isolation and Sequencing
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DNA Isolation and Sequencing, DNA isolation and sequencing are core activities in molecular biology. This concise protocol book provides comprehensive practical guidance on the major methods available, from extraction and restriction digestion, to small and large scale isolation, sequ, DNA Isolation and Sequencing
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  • DNA Isolation and Sequencing
  • Written by author Bruce A. Roe
  • Published by Wiley-Blackwell, 1996/05/09
  • DNA isolation and sequencing are core activities in molecular biology. This concise protocol book provides comprehensive practical guidance on the major methods available, from extraction and restriction digestion, to small and large scale isolation, sequ
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Partial table of contents:

GENERAL METHODS.

Restriction Digestion.

Agarose Gel Electrophoresis.

Bacterial Cell Maintenance and Storage.

RANDOM SUBCLONE GENERATION.

Sonication.

DNA Ligation.

METHODS FOR DNA ISOLATION.

Large Scale Double-Stranded DNA Isolation.

Miniprep Double-Stranded DNA Isolation.

METHODS FOR DNA SEQUENCING.

Radiolabeled Sequencing Gel Preparation, Loading and Electrophoresis.

Flourescent DNA Sequencing.

ADDITIONAL METHODS.

Polymerase Chain Reaction (PCR).

Purification of PCR Fragments for Cloning.

Synthesis and Purification of Oligonucleotides.

Appendices.

Index.


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DNA Isolation and Sequencing, DNA isolation and sequencing are core activities in molecular biology. This concise protocol book provides comprehensive practical guidance on the major methods available, from extraction and restriction digestion, to small and large scale isolation, sequ, DNA Isolation and Sequencing

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DNA Isolation and Sequencing, DNA isolation and sequencing are core activities in molecular biology. This concise protocol book provides comprehensive practical guidance on the major methods available, from extraction and restriction digestion, to small and large scale isolation, sequ, DNA Isolation and Sequencing

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DNA Isolation and Sequencing, DNA isolation and sequencing are core activities in molecular biology. This concise protocol book provides comprehensive practical guidance on the major methods available, from extraction and restriction digestion, to small and large scale isolation, sequ, DNA Isolation and Sequencing

DNA Isolation and Sequencing

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