Contemporary Enzyme Kinetics and Mechanism Book

	List of Contributors
	Preface
	Contents of Methods in Enzymology, Volumes 63, 64, 87, and 249
1	Transient Kinetic Approaches to Enzyme Mechanisms	1
2	Practical Considerations in the Design of Initial Velocity Enzyme Rate Assays	37
3	Derivation of Initial Velocity and Isotope Exchange Rate Equations	57
4	Plotting Methods for Analyzing Enzyme Rate Data	89
5	Regression Analysis, Experimental Error, and Statistical Criteria in the Design and Analysis of Experiments for Discrimination between Rival Kinetic Models	111
6	Cooperativity in Enzyme Function: Equilibrium and Kinetic Aspects	133
7	Product Inhibition Applications	183
8	Reversible Enzyme Inhibitors as Mechanistic Probes	207
9	Transition State and Multisubstrate Analog Inhibitors	229
10	Application of Affinity Labeling for Studying Structure and Function of Enzymes	259
11	Mechanism Based Enzyme Inactivators	291
12	Isotope Exchange Methods for Elucidating Enzymic Catalysis	335
13	Positional Isotope Exchange as Probe of Enzyme Action	379
14	Isotope Effects: Determination of Enzyme Transition State Structure	407
15	Hydrogen Tunneling in Enzyme Catalysis	441
16	Site-Directed Mutagenesis: A Tool for Studying Enzyme Catalysis	467
17	Selected Exercises and Problems	497
	Index	531