Classic experiments in modern biology Book

	Preface
1	The Power of Microscopy	1
1.1	Basic Principles of Microscopy	1
1.2	Seeing Signs of Disease	5
1.3	The Fluid Mosaic Model of Membrane Structure	13
1.4	Packing DNA into Chromatin and Chromosomes	23
	Discussion Questions	42
	References	42
2	Purification and Properties of Protein Kinase	45
2.1	Protein Purification Techniques	45
2.2	Purification of Nuclear Protein Kinase	50
2.3	Control of Enzyme Activity	61
2.4	Identification of the Protein Encoded by a Specific Gene	65
2.5	The Relation of Protein Kinases to Evolution	70
	Discussion Questions	72
	References	73
3	The Discovery and Properties of Messenger RNA	75
3.1	Genes and Protein Synthesis	75
3.2	The Discovery of T2 Messenger RNA	77
3.3	Properties of Prokaryotic Messenger RNA	88
3.4	Eukaryotic Messenger RNA	93
	Discussion Questions	105
	References	105
4	Cell Differentiation, Aging, and Cancer: Cell Culture Studies	107
4.1	Cellular Differentiation in Vitro	109
4.2	Cellular Aging, Immortalization, and Cancer	121
	Discussion Questions	137
	References	137
5	Genetic Engineering: Techniques and Applications	141
5.1	Genetic Tools	142
5.2	Important Techniques for Cloning Genes	148
5.3	Gene Cloning and Diabetes	156
5.4	Other Applications of Genetic Engineering Technology	167
	Discussion Questions	174
	References	174
	Appendix 1. Techniques and Procedures	177
	Appendix 2. Abbreviations and Acronyms	179
	Glossary	181
	Credits	193