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cDNA Library Protocols, Vol. 69 Book

cDNA Library Protocols, Vol. 69
cDNA Library Protocols, Vol. 69, This comprehensive collection of detailed prools covers all areas of cDNA work, from library construction and manipulation to screening and analysis of resulting clones. Great care has been taken to combine up-to-date versions of some of the most widely u, cDNA Library Protocols, Vol. 69 has a rating of 3 stars
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cDNA Library Protocols, Vol. 69, This comprehensive collection of detailed prools covers all areas of cDNA work, from library construction and manipulation to screening and analysis of resulting clones. Great care has been taken to combine up-to-date versions of some of the most widely u, cDNA Library Protocols, Vol. 69
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  • cDNA Library Protocols, Vol. 69
  • Written by author Ian G. Cowell
  • Published by Springer-Verlag New York, LLC, December 2009
  • This comprehensive collection of detailed prools covers all areas of cDNA work, from library construction and manipulation to screening and analysis of resulting clones. Great care has been taken to combine up-to-date versions of some of the most widely u
  • This comprehensive collection of detailed protocols covers all areas of cDNA work, from library construction and manipulation to screening and analysis of resulting clones. Great care has been taken to combine up-to-date versions of some of the most widel
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Authors

cDNA Library Construction from Small Amounts of RNA Using Paramagnetic Beads and PCR

Kris N. Lambert and Valerie M. Williamson

Increasing the Average Abundance of Low-Abundance cDNAs by Ordered Subdivision of cDNA Populations

David R. Sibson and Michael P. Starkey

Isolation of Messenger RNA from Plant Tissues

Alison Dunn

cDNA Library Construction for the Lambda ZAP®-Based Vectors

Marjory A. Snead, Michelle A. Alting-Mees, and Jay M. Short

Clone Excision Methods for the Lambda ZAP®-Based Vectors

Marjory A. Snead, Michelle A. Alting-Mees, and Jay M. Short

Using Rapid Amplification of cDNA Ends (RACE) to Obtain Full-Length cDNAs

Yue Zhang and Michael A. Frohman

Inverse PCR Approach to Cloning cDNA Ends

Sheng-He Huang

Cloning Gene Family Members Using the Polymerase Chain Reaction with Degenerate Oligonucleotide Primers

Gregory M. Preston

Subtractive Hybridization for the Isolation of Differentially Expressed Genes Using Magnetic Beads

Hans-Christian Aasheim, Ton Logtenberg, and Frank Larsen

Preparation of Competent Cells for High-Efficiency Plasmid Transformation of Escherichia coli

Yasuhiko Nakata, Xiaoren Tang, and Kazunari K. Yokoyama

The Tetramethylammonium Chloride (TMAC) Method for Screening cDNA Libraries with Highly Degenerate Oligonucleotide Probes Obtained by Reverse Translation of Amino Acid Sequences

Bent Honoré and Peder Madsen

Screening cDNA Libraries by Hybridization with Double-Stranded DNA Probes and Oligonucleotides

Caroline A. Austin

Immunological Screening of l-Phage cDNA Expression Libraries

Helen C. Hurst

Cloning Sequence-Specific DNA-Binding Factors from cDNA Expression Libraries Using Oligonucleotide Binding Site Probes

Ian G. Cowell

Protein Interaction Cloning by Far-Western Screening of l-Phage cDNA Expression Libraries

Shinichi Takayama and John C. Reed

Yeast Two-Hybrid Library Screening

Ian G. Cowell

Signal Sequence Trap: Expression Cloning Method for Secreted Proteins and Type 1 Membrane Proteins

Kei Tashiro, Toru Nakano, and Tasuku Honjo

Isolation of Genetic Suppressor Elements (GSEs) from Random Fragment cDNA Libraries in Retroviral Vectors

Andrei V. Gudkov and Igor B. Roninson

Expression and Preparation of Fusion Proteins from Recombinant lgt11 Phages

Sheng-He Huang and Ambrose Jong

Computer Analysis of Cloned Sequences

Paul R. Caron

The TIGR Human cDNA Database

Ewen F. Kirkness and Anthony R. Kerlavage

Searching the dbEST Database

Patricia Rodriguez Tomé

Reference cDNA Library Facilities Available from European Sources

Michael P. Starkey, Yagnesh Umrania, Christopher R. Mundy, and Martin J. Bishop

Index


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cDNA Library Protocols, Vol. 69, This comprehensive collection of detailed prools covers all areas of cDNA work, from library construction and manipulation to screening and analysis of resulting clones. Great care has been taken to combine up-to-date versions of some of the most widely u, cDNA Library Protocols, Vol. 69

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cDNA Library Protocols, Vol. 69, This comprehensive collection of detailed prools covers all areas of cDNA work, from library construction and manipulation to screening and analysis of resulting clones. Great care has been taken to combine up-to-date versions of some of the most widely u, cDNA Library Protocols, Vol. 69

cDNA Library Protocols, Vol. 69

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cDNA Library Protocols, Vol. 69, This comprehensive collection of detailed prools covers all areas of cDNA work, from library construction and manipulation to screening and analysis of resulting clones. Great care has been taken to combine up-to-date versions of some of the most widely u, cDNA Library Protocols, Vol. 69

cDNA Library Protocols, Vol. 69

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