Cardiovascular Proteomics: Methods and Protocols Book

Part I. Analysis of Heart Proteome

Two-dimensional Polyacrylamide Gel Electrophoresis for Cardiovascular Proteomics

Michael J. Dunn

Analyzing The Cardiac Muscle Proteome By Liquid Chromatography-Mass Spectrometry (Lc-ms) Based Expression Proteomics

Anthony O. Gramolini, Thomas Kislinger, Peter Liu, David H. Maclennan, and Andrew Emili

2-D Differential Gel Electrophoresis (DIGE) of Rat Heart Proteins in Ischemia and Ischemia-Reperfusion

Jun Sakai, Hironori Ishikawa, Hideshi Satoh, Setsuko Yamamoto, Shinichi Kojima, and Masaharu Kanoaka

Analysis of Antihypertensive Drugs in the Heart of Animal Models: A Proteomic Approach

Alberto Lázaro, Julio Gállego-delgado, Julio Osende, Jes&u's Egido, and Fernando Vivanco

A Solubility Optimization Prool for 2-D Gel Electrophoresis of Cardiac Tissue

Brian A. Stanley and Jennifer E. Van Eyk

A Method for Effective Depletion of Albumin From Cellular Extracts: Application to Human Myocardium

Brian A. Stanley and Jennifer E. Van Eyk

Part II. Heart Subproteomes

Subcellular Fractionation

Mariana E. Guimarães De Araújo and Alfons Huber

Subfractionation of Heart Tissue: The "In Sequence" Myofilament Protein Extraction Of Myocardial Tissue

Lesley A. Kane, Irina Neverova, and Jennifer E. Van Eyk

Optimization of Cardiac Troponin I Pull-Down by Idm Affinity Beads and Seldi-tofT

Diane E. Bovenkamp, Brian A. Stanley and Jennifer E.van Eyk

Proteomic Analysis Of The Subunit Composition of Complex I (Nadh:Ubiquinone Oxidoreductase) From Bovine Heart Mihondria

Ian M. Fearnley, Joe Carrol and John E. Walker

Identification of Targets of Phosphorylation in Heart Mihondria

Roberta A. Gottliebie

Part III. Proteomics of Human Atheroma Plaques

Characterization of The Human Atheroma Plaque Secretome by Proteomic Analysis

Mari-Carmen Durán, Jose L. Martin-Ventura, Sebastián Mas, Maria G. Barderas, Verónica M. Dardé, Ole N. Jensen, Jes&u's Egido, and Fernando Vivanco

Characterization of Hsp-27 Phosphorylation Sites in Human Atherosclerotic Plaque Secretome

Mari-Carmen Durán, Elisabetta Boeri-Erba, Shabaz Mohammed, Jose L. Martín-Ventura, Jes&u's Egido, Fernando Vivanco, and Ole N. Jensen

Western Array Analysis of Human Atherosclerotic Plaque

Wim Martinetar

Part IV. The Proteome of Cells of The Cardiovascular System

The Proteome of Endothelial Cells

Jes&u's Gonzalez-Cabrero, Mayte Pozo, Mari-Carmen Durán, Rosario de Nicolás, Jes&u's Egido, and Fernando Vivanco

Proteomic Study of Caveolae and Rafts Isolated from Human Endothelial Cells

Richard R. Sprenger and Anton J. G. Horrevoets

Proteomic Identification of S-nitrosylated Proteins In Endothelial Cells

Antonio Martínez-Ruiz and Santiago Lamasgo

The Proteome and Secretome of Human Arterial Smooth Muscle Cell

Annabelle Dupont and Florence Pinet

Real-Time in Vivo Proteomic Identification Of Novel Kinase Substrates in Smooth Muscle

Anne A. Wooldridge and Timothy A. Haystead

Proteomic Analysis of Vascular Smooth Muscle Cells Treated With Ouabain

Alexy V. Pshezhetsky

Isolation and Culture of Adult Mouse Cardiac Myocytes

Timothy D. ÓConnell, Manoj C. Rodrigo, and Paul C. Simpson

Proteomic Analysis Of Foam Cells

Peng-Yuang Yang, Yao-cheng Rui, Peng-Yuan Yang, and Yan-Ling Yuin

Isolation of The Platelet Releasate

Judith Coppinger, Desmond J. Fitzgerald, and Patricia B. Maguire

Enrichment Of Phospho-Tyrosine Proteome Of Human Platelets By Immunoprecipitation

Martina Foy, Donald F. Harney, Kieran Wynne, and Patricia B. Maguire

Chapter 25

Verónica M. Dardé, Mari-Carmen Durán, Jes&u's Egido, and Fernando Vivanco

Part V. Cardiovascular Biomarkers in Plasma

Cardiovascular Biomarkers Discovery By Seldi-tof Mass Spectrometry

Olivier Meilhac, Sandrine Delbosc, and Jean-Baptiste Michel

Detection of Biomarkers of Stroke Using Seldi-tofe

Jean-charles Sanchez, Pierre Lescuyer, Denis Hochstrasser, and Laure Allard

Depletion of High-Abundance Proteins In Plasma by Immunoaffinity Substraction For Two-Dimensional Difference Gel Electrophoresis (DIGE) Analysis

Verónica M. Dardé, Maria G. Barderas, and Fernando Vivanco

A Rapid, Economic and Reproducible Method For Human Serum Delipidation, Albumin And IgG Removal, For Proteomic Analysis

Qin Fu, Diane E. Bovenkamp And Jennifer E.van Eyk

Part VI. Bioinformatics: Emerging Technologies

Mitoproteome: Human Heart Mihondrial Protein Sequence Database

Purnima Guda, Shankar Subramaniam, and Chittibabu Guda

Techniques To Decipher Molecular Diversity By Phage Display

Dawn R. Christianson, Michael G. Ozawa, Renata Pasqualini, and Wadih Arapraprap