Brain Neurosecretory Cytokines: Immune Response and Neuronal Survival Book

Ch. 1	Neurosecretion of cytokines by N. paraventricularis and N. supraopticus of hypothalamus	5
1.1	The isolation of neurosecretory granules from bovine hypothalamus and neurohypophysis : bioassay methods of cytokine identification	5
1.2	Identification of interleukins 1[alpha] and 1[beta] in the lysates of neurosecretory granules of bovine neurohypophysis and hypothalamus	6
1.3	Identification of IL-2 in the neurosecretory granules of bovine neurohypophysis and hypothalamus	8
1.4	Identification of IL-6 in the neurosecretory granules of bovine neurohypophysis	9
1.5	Identification of tumor necrosis factor [alpha] (TNF-[alpha]) in the neurosecretory granules of bovine hypothalamus and neurohypophysis	9
Ch. 2	Discovery of new neurosecretory cytokines : prolinerich peptides produced by N. paraventricularis and N. supraopticus of hypothalamus	13
2.1	Isolation of proline-rich peptides from the neurosecretory granules of bovine neurohypophysis : primary structures, mass-spectral characteristics, and localization	13
2.2	Identification of PRP-1 in the brain by immunohistochemical methods using polyclonal antibodies	23
2.3	Determination of PRP-1 in the thymus, lymph nodes, and heart using monoclonal antibodies	27
2.4	Interactions of PRP-1 with coagulation cascade components : a potential role for PRP-1 in the metabolism of factor Xa/antithrombin III complex	31
Ch. 3	Interaction of PRP-1 with cytokines, hormones, and neurotransmitters	33
3.1	Effect of PRP-1 and lysates of neurosecretory granules on the expression of cytokines in macrophages and astrocytes	33
3.2	Effect of PRP-1 on the expression of interleukins and prolactin in different organs of mice during aluminum neurotoxicosis	37
3.3	PRP-1 increases the expression and release of human growth hormone in the culture of BALB/c mouse fibroblasts	40
Ch. 4	Antibacterial and antiviral activity of PRP-1 and some immunological mechanisms of its action	45
4.1	Effects of PRP-1 on the survival of mice infected with different strains of gram-negative and gram-positive bacteria at lethal doses	46
4.2	Effects of PRP-1 on the growth of bacteria in the internal organs of mice infected with S. typhimurium	47
4.3	Effects of PRP-1 on anti-microbial antibody production in mice infected with S. typhimurium and S. cholerae suis in lethal doses	47
4.4	Effect of PRP-1 on bactericidal activity of macrophages	48
4.5	Effect of PRP-1 on the secretion of interleukin-1 by peritoneal macrophages of mice infected with S. typhimurium in sublethal doses	48
4.6	Effect of PRP-1 on the antigen-presenting function of macrophages in mice infected with S. typhimurium	49
4.7	Effect of PRP-1 on the accumulation of macrophages in peritoneal cavities of mice infected with S. typhimurium	50
4.8	Effect of PRP-1 on the viability of macrophages in mice infected with S. typhimurium	50
4.9	Effect of PRP-1 on interferon-[gamma] (IFN-[gamma]) biosynthesis in human mononuclear cells and the replication of encephalomyocarditis virus in cell culture	53
Ch. 5	PRP regulation of T-lymphocyte development and myelopoiesis	57
5.1	Influence of PRP-1 on interleukin-2-dependent functions of human lymphocytes in culture and on the proliferation of jurkat cells	57
5.2	Regulation of thymocyte differentiation by PRP-1 in neonatal and fetal thymus	60
5.3	PRP-1 is a regulator of myelopoiesis	66
Ch. 6	Neuroprotective (antineurodegenerative) properties of PRP-1	75
6.1	Protective effect of PRP-1 against snake venom-induced neuronal injury	75
6.2	Protective effect of PRP-1 against trauma-induced central and peripheral neuronal injury	93
6.3	Morphological and biochemical changes in brain microstructure in crush syndrome and effect of PRP-1	110
6.4	PRP-1 protects brain neurons against aluminum (Al) neurotoxicosis (electron microscopy studies)	118
Ch. 7	Effect of PRP-1 on tumor cells	129
7.1	Effect of PRP-1 on the morphology and mitotic activity of neurinoma of rat noduli gasser (NRNG) cells in culture (electron microscopy studies)	130
7.2	Changes in the ultrastructure of L[subscript 929] tumor cells under the action of PRP-1 in vitro	133
Ch. 8	Basic biochemical mechanisms of PRP-1 action in different pathologies	137
8.1	Effect of PRP-1 on GFAP biosynthesis in astrocyte culture (immunocytochemical data)	137
8.2	Effect of PRP-1 on caspase activity of murine neuroblastoma N2A	140
8.3	Regulation of membrane phospholipid metabolism by PRP-1 in cardiopulmonary insufficiency	144
8.4	Influence of PRP-1 on consequences of ionizing irradiation in rats	153