Basic Plant Pathology Methods Book

Sterilization of Apparatus and Culture Media Heat Sterilization Gas Sterilization Filtration Culture of Pathogens Factors Affecting the Cultivation and Sporulation of Pathogenic Fungi Some Special Methods of Inducing Sporulation in Fungi Cultivation of Pathogens for Soil Infestation Other Sources of Inoculum Cultivation and Sporulation of Some Pathogens Long-Term Storage of Plant Pathogens Periodic Transfer Mineral Oil Drying Storage in Water Storage in Silica Gel Storage in Soil Freezing Preservation in Liquid Nitrogen Lyophilization and Vacuum Drying Detection and Estimation of Inoculum Soilborne Pathogens Detection and Estimation of Inoculum in the Air Detection of Pathogens Associated with Seeds Establishment of Disease and Testing for Resistance Factors Influencing Disease Establishment Root Inoculation Stem Inoculation Leaf Inoculation Inoculation of Cereal Inflorescences Seed Inoculation Inoculation with Bacteria Inoculation of Detached Plant Parts Field Inoculation Methods Some Special Hints Soil Microorganisms Isolation and Enumeration of Soil Organisms Observation of Soil Microorganisms in Situ Physiological Processes as an Estimate of Biological Activity in Soil Spore Germination in Soil and Soil Fungistasis Survival of Plant Pathogens in Soil Mycelial Growth in Soil Collection of Root and Seed Exudates Artificial Rhizosphere or Spermosphere Fungicide Evaluation Spore Germination Test Tests Based on Growth on Organisms In Vitro Transfer-Bioassay Techniques Laboratory Testing for Soil Fungicides Greenhouse Testing of Foliar Fungicides Greenhouse Testing of Fungicides for Control of Soilborne Pathogens Planning Field Experiments Seed Treatment with Chemicals Biological Control Collection and Testing of Soil Samples for Antagonists Isolation of Antibiotic-Producing Organisms Isolation and Testing of Lytic Organisms Isolation and Testing of Mycoparasitic Organisms Spore Perforating Amoebae Isolation of Antagonists from Leaves Testing Antagonists for Biological Control Bright-Field Microscopy Techniques Mounting and Staining of Fungi for Microscopic Examination Staining of Fungal Nucelli Mounting Spores for Viewing from Different Positions Immobilization of Fungus Spores in Water Mounts for Microscopic Examination Staining of Bacteria Cements for Sealing Mounts Slide Culture Techniques and Preparation of Permanent Mounts Preparing Agar-Colony Mounts and Dry Reference Cultures Examination of Host-Parasite Relationship Using Bright-Field Microscopy Appendices Culture Media and Their Formulas Plant Nutrient Solutions Solutions to Maintain Constant Humidity in a Closed Atmosphere Drying Agents pH Indicator Dyes Approximate Figures for Concentrated Acids and Bases Preservation of Museum Specimens Collection, Preparation, and Mailing of Cultures and Specimens Glassware Cleaning Solutions Index