Basic Cell Culture Protocols Book

1. Detection of Mycoplasma Contaminations
Cord C. Uphoff and Hans G. Drexler

2. Eradication of Mycoplasma Contaminations
Cord C. Uphoff and Hans G. Drexler

3. STR DNA Typing of Human Cell Lines: Detection of Intra- and Interspecies Cross-Contamination Wilhelm G. Dirks and Hans G. Drexler

4. Classical and Molecular Cytogenetic Analysis Roderick A.F. MacLeod and Hans G. Drexler

5. Fluorescent In Situ Hybridization (FISH) of DNA Probes in the Interphase and Metaphase Stages of the Cell Cycle
Linda A. Cannizzaro

6. The Development of T Lymphocytes in Fetal Thymus Organ Culture
Takeshi Nitta, Izumi Ohigashi, and Yousuke Takahama

7. Generation, Isolation, and Engraftment of In Vitro-Derived Human T Cell Progenitors
Génève Awong and Juan Carlos Zúñiga-Pflücker

8. In Vitro Generation of Human T Regulatory Cells: Generation, Culture, and Analysis of FOXP3-Transduced T Cells
Alicia N. McMurchy and Megan K. Levings

9. Simultaneous Cloning and Selection of Hybridomas and Transfected Cell Lines in Semisolid Media Bert Wognum and Tracy Lee

10. Isolation and Characterization of Mouse Side Population Cells Aysegul V. Ergen, Mira Jeong, Kuanyin K. Lin, Grant A. Challen, and Margaret A. Goodell

11. Stem Cell Identification by DyeCycle Violet Side Population Analysis William G. Telford

12. Isolation and Characterization of Cancer Stem Cells In Vitro Craig Gedye and Laurie Ailles

13. Ex Vivo Differentiation of Cord Blood Stem Cells into Megakaryocytes and Platelets Nicolas Pineault, Amélie Robert, Valérie Cortin, and Lucie Boyer

14. Generation and Characterization of Murine Alternatively Activated Macrophages Shelley B. Weisser, Keith W. McLarren, Etsushi Kuroda, and Laura M. Sly
15. Human Long Term Culture Initiating Cell (LTC-IC) Assay Min Liu, Cindy Miller, and Connie Eaves

16. Long Term Culture Initiating Cell (LTC-IC) Assay for Mouse Cells Stefan Wohrer, Cindy L. Miller, and Connie J Eaves

17. Colony Forming Cell Assays for Human Hematopoietic Progenitor Cells Bert Wognum, Ning Yuan, Becky Lai, and Cindy L. Miller

18. Studying Leukocyte Recruitment Under Flow Conditions Sean A. Parsons, Christophe Jurzinsky, Susan L. Cuvelier, and Kamala D. Patel

19. Generation and Establishment of Murine Adherent Cell Lines Rouzanna Istvanffy and Robert A.J. Oostendorp

20. Isolation, Enumeration, and Expansion of Human Mesenchymal Stem Cells in Culture Ravenska Wagey and Brenton Short

21. Isolation and Culture of Mesenchymal Stem Cells from Mouse Compact Bone Brenton Short and Ravenska Wagey

22. Generation of a Pool of Human Platelet Lysate (pHPL) and Efficient Use in Cell Culture Katharina Schallmoser and Dirk Strunk

23. In Vitro Methods to Culture Primary Human Breast Epithelial Cells Afshin Raouf and Yu Jia Sun

24. Human Prostate Epithelial Cell Cultures Johng S. Rhim

25. Enzymatic Dissociation, Flow Cytometric Analysis, and Culture of Normal Mouse Mammary Tissue Michael Prater, Mona Shehata, Christine J. Watson, and John Stingl

26. Isolation and Characterization of Human Hair Follicle Epithelial Cells Keita Inoue and Kotaro Yoshimura

27. Co-Cultivation of Human Oral Keratinocytes and Human Osteoblast-Like Cells Ricarda Glaum and Margit Wiedmann-Al-Ahmad
28. Isolation and Culture of Skeletal Muscle Myofibers as a Means to Analyze Satellite Cells Paul Keire, Andrew Shearer, Gabi Shefer, and Zipora Yablonka-Reuveni

29. Hepatic Differentiation of Embryonic Stem Cells by Murine Fetal Liver Mesenchymal Cells Takamichi Ishii, Kentaro Yasuchika, and Iwao Ikai

30. Methods to Culture, Differentiate, and Characterize Neural Stem Cells from the Adult and Embryonic Mouse Central Nervous System Sharon A. Louis, Carmen K.H. Mak, and Brent A. Reynolds

31. Feeder-Independent Culture Systems for Human Pluripotent Stem Cells
Jennifer Moody

32. Formation of Embryoid Bodies from Human Pluripotent Stem Cells Using AggreWell™ Plates Jennifer Antonchuk

33. Techniques in Embryoid Body Formation from Human Pluripotent Stem Cells
Nirupama K. Shevde and Amber A. Mael

Wilhelm G. Dirks and Hans G. Drexler

4. Classical and Molecular Cytogenetic Analysis Roderick A.F. MacLeod and Hans G. Drexler

5. Fluorescent In Situ Hybridization (FISH) of DNA Probes in the Interphase and Metaphase Stages of the Cell Cycle
Linda A. Cannizzaro

6. The Development of T Lymphocytes in Fetal Thymus Organ Culture
Takeshi Nitta, Izumi Ohigashi, and Yousuke Takahama

7. Generation, Isolation, and Engraftment of In Vitro-Derived Human T Cell Progenitors
Génève Awong and Juan Carlos Zúñiga-Pflücker

8. In Vitro Generation of Human T Regulatory Cells: Generation, Culture, and Analysis of FOXP3-Transduced T Cells
Alicia N. McMurchy and Megan K. Levings

9. Simultaneous Cloning and Selection of Hybridomas and Transfected Cell Lines in Semisolid Media Bert Wognum and Tracy Lee

10. Isolation and Characterization of Mouse Side Population Cells Aysegul V. Ergen, Mira Jeong, Kuanyin K. Lin, Grant A. Challen, and Margaret A. Goodell

11. Stem Cell Identification by DyeCycle Violet Side Population Analysis William G. Telford

12. Isolation and Characterization of Cancer Stem Cells In Vitro Craig Gedye and Laurie Ailles

13. Ex Vivo Differentiation of Cord Blood Stem Cells into Megakaryocytes and Platelets Nicolas Pineault, Amélie Robert, Valérie Cortin, and Lucie Boyer

14. Generation and Characterization of Murine Alternatively Activated Macrophages Shelley B. Weisser, Keith W. McLarren, Etsushi Kuroda, and Laura M. Sly
15. Human Long Term Culture Initiating Cell (LTC-IC) Assay Min Liu, Cindy Miller, and Connie Eaves

16. Long Term Culture Initiating Cell (LTC-IC) Assay for Mouse Cells Stefan Wohrer, Cindy L. Miller, and Connie J Eaves

17. Colony Forming Cell Assays for Human Hematopoietic Progenitor Cells Bert Wognum, Ning Yuan, Becky Lai, and Cindy L. Miller

18. Studying Leukocyte Recruitment Under Flow Conditions Sean A. Parsons, Christophe Jurzinsky, Susan L. Cuvelier, and Kamala D. Patel

19. Generation and Establishment of Murine Adherent Cell Lines Rouzanna Istvanffy and Robert A.J. Oostendorp

20. Isolation, Enumeration, and Expansion of Human Mesenchymal Stem Cells in Culture Ravenska Wagey and Brenton Short

21. Isolation and Culture of Mesenchymal Stem Cells from Mouse Compact Bone Brenton Short and Ravenska Wagey

22. Generation of a Pool of Human Platelet Lysate (pHPL) and Efficient Use in Cell Culture Katharina Schallmoser and Dirk Strunk

23. In Vitro Methods to Culture Primary Human Breast Epithelial Cells Afshin Raouf and Yu Jia Sun

24. Human Prostate Epithelial Cell Cultures Johng S. Rhim

25. Enzymatic Dissociation, Flow Cytometric Analysis, and Culture of Normal Mouse Mammary Tissue Michael Prater, Mona Shehata, Christine J. Watson, and John Stingl

26. Isolation and Characterization of Human Hair Follicle Epithelial Cells Keita Inoue and Kotaro Yoshimura

27. Co-Cultivation of Human Oral Keratinocytes and Human Osteoblast-Like Cells Ricarda Glaum and Margit Wiedmann-Al-Ahmad
28. Isolation and Culture of Skeletal Muscle Myofibers as a Means to Analyze Satellite Cells Paul Keire, Andrew Shearer, Gabi Shefer, and Zipora Yablonka-Reuveni

29. Hepatic Differentiation of Embryonic Stem Cells by Murine Fetal Liver Mesenchymal Cells Takamichi Ishii, Kentaro Yasuchika, and Iwao Ikai

30. Methods to Culture, Differentiate, and Characterize Neural Stem Cells from the Adult and Embryonic Mouse Central Nervous System Sharon A. Louis, Carmen K.H. Mak, and Brent A. Reynolds

31. Feeder-Independent Culture Systems for Human Pluripotent Stem Cells
Jennifer Moody

32. Formation of Embryoid Bodies from Human Pluripotent Stem Cells Using AggreWell™ Plates Jennifer Antonchuk

33. Techniques in Embryoid Body Formation from Human Pluripotent Stem Cells
Nirupama K. Shevde and Amber A. Mael

Roderick A.F. MacLeod and Hans G. Drexler

5. Fluorescent In Situ Hybridization (FISH) of DNA Probes in the Interphase and Metaphase Stages of the Cell Cycle
Linda A. Cannizzaro

6. The Development of T Lymphocytes in Fetal Thymus Organ Culture
Takeshi Nitta, Izumi Ohigashi, and Yousuke Takahama

7. Generation, Isolation, and Engraftment of In Vitro-Derived Human T Cell Progenitors
Génève Awong and Juan Carlos Zúñiga-Pflücker

8. In Vitro Generation of Human T Regulatory Cells: Generation, Culture, and Analysis of FOXP3-Transduced T Cells
Alicia N. McMurchy and Megan K. Levings

9. Simultaneous Cloning and Selection of Hybridomas and Transfected Cell Lines in Semisolid Media Bert Wognum and Tracy Lee

10. Isolation and Characterization of Mouse Side Population Cells Aysegul V. Ergen, Mira Jeong, Kuanyin K. Lin, Grant A. Challen, and Margaret A. Goodell

11. Stem Cell Identification by DyeCycle Violet Side Population Analysis William G. Telford

12. Isolation and Characterization of Cancer Stem Cells In Vitro Craig Gedye and Laurie Ailles

13. Ex Vivo Differentiation of Cord Blood Stem Cells into Megakaryocytes and Platelets Nicolas Pineault, Amélie Robert, Valérie Cortin, and Lucie Boyer

14. Generation and Characterization of Murine Alternatively Activated Macrophages Shelley B. Weisser, Keith W. McLarren, Etsushi Kuroda, and Laura M. Sly
15. Human Long Term Culture Initiating Cell (LTC-IC) Assay Min Liu, Cindy Miller, and Connie Eaves

16. Long Term Culture Initiating Cell (LTC-IC) Assay for Mouse Cells Stefan Wohrer, Cindy L. Miller, and Connie J Eaves

17. Colony Forming Cell Assays for Human Hematopoietic Progenitor Cells Bert Wognum, Ning Yuan, Becky Lai, and Cindy L. Miller

18. Studying Leukocyte Recruitment Under Flow Conditions Sean A. Parsons, Christophe Jurzinsky, Susan L. Cuvelier, and Kamala D. Patel

19. Generation and Establishment of Murine Adherent Cell Lines Rouzanna Istvanffy and Robert A.J. Oostendorp

20. Isolation, Enumeration, and Expansion of Human Mesenchymal Stem Cells in Culture Ravenska Wagey and Brenton Short

21. Isolation and Culture of Mesenchymal Stem Cells from Mouse Compact Bone Brenton Short and Ravenska Wagey

22. Generation of a Pool of Human Platelet Lysate (pHPL) and Efficient Use in Cell Culture Katharina Schallmoser and Dirk Strunk

23. In Vitro Methods to Culture Primary Human Breast Epithelial Cells Afshin Raouf and Yu Jia Sun

24. Human Prostate Epithelial Cell Cultures Johng S. Rhim

25. Enzymatic Dissociation, Flow Cytometric Analysis, and Culture of Normal Mouse Mammary Tissue Michael Prater, Mona Shehata, Christine J. Watson, and John Stingl

26. Isolation and Characterization of Human Hair Follicle Epithelial Cells Keita Inoue and Kotaro Yoshimura

27. Co-Cultivation of Human Oral Keratinocytes and Human Osteoblast-Like Cells Ricarda Glaum and Margit Wiedmann-Al-Ahmad
28. Isolation and Culture of Skeletal Muscle Myofibers as a Means to Analyze Satellite Cells Paul Keire, Andrew Shearer, Gabi Shefer, and Zipora Yablonka-Reuveni

29. Hepatic Differentiation of Embryonic Stem Cells by Murine Fetal Liver Mesenchymal Cells Takamichi Ishii, Kentaro Yasuchika, and Iwao Ikai

30. Methods to Culture, Differentiate, and Characterize Neural Stem Cells from the Adult and Embryonic Mouse Central Nervous System Sharon A. Louis, Carmen K.H. Mak, and Brent A. Reynolds

31. Feeder-Independent Culture Systems for Human Pluripotent Stem Cells
Jennifer Moody

32. Formation of Embryoid Bodies from Human Pluripotent Stem Cells Using AggreWell™ Plates Jennifer Antonchuk

33. Techniques in Embryoid Body Formation from Human Pluripotent Stem Cells
Nirupama K. Shevde and Amber A. Mael

Bert Wognum and Tracy Lee

10. Isolation and Characterization of Mouse Side Population Cells Aysegul V. Ergen, Mira Jeong, Kuanyin K. Lin, Grant A. Challen, and Margaret A. Goodell

11. Stem Cell Identification by DyeCycle Violet Side Population Analysis William G. Telford

12. Isolation and Characterization of Cancer Stem Cells In Vitro Craig Gedye and Laurie Ailles

13. Ex Vivo Differentiation of Cord Blood Stem Cells into Megakaryocytes and Platelets Nicolas Pineault, Amélie Robert, Valérie Cortin, and Lucie Boyer

14. Generation and Characterization of Murine Alternatively Activated Macrophages Shelley B. Weisser, Keith W. McLarren, Etsushi Kuroda, and Laura M. Sly
15. Human Long Term Culture Initiating Cell (LTC-IC) Assay Min Liu, Cindy Miller, and Connie Eaves

16. Long Term Culture Initiating Cell (LTC-IC) Assay for Mouse Cells Stefan Wohrer, Cindy L. Miller, and Connie J Eaves

17. Colony Forming Cell Assays for Human Hematopoietic Progenitor Cells Bert Wognum, Ning Yuan, Becky Lai, and Cindy L. Miller

18. Studying Leukocyte Recruitment Under Flow Conditions Sean A. Parsons, Christophe Jurzinsky, Susan L. Cuvelier, and Kamala D. Patel

19. Generation and Establishment of Murine Adherent Cell Lines Rouzanna Istvanffy and Robert A.J. Oostendorp

20. Isolation, Enumeration, and Expansion of Human Mesenchymal Stem Cells in Culture Ravenska Wagey and Brenton Short

21. Isolation and Culture of Mesenchymal Stem Cells from Mouse Compact Bone Brenton Short and Ravenska Wagey

22. Generation of a Pool of Human Platelet Lysate (pHPL) and Efficient Use in Cell Culture Katharina Schallmoser and Dirk Strunk

23. In Vitro Methods to Culture Primary Human Breast Epithelial Cells Afshin Raouf and Yu Jia Sun

24. Human Prostate Epithelial Cell Cultures Johng S. Rhim

25. Enzymatic Dissociation, Flow Cytometric Analysis, and Culture of Normal Mouse Mammary Tissue Michael Prater, Mona Shehata, Christine J. Watson, and John Stingl

26. Isolation and Characterization of Human Hair Follicle Epithelial Cells Keita Inoue and Kotaro Yoshimura

27. Co-Cultivation of Human Oral Keratinocytes and Human Osteoblast-Like Cells Ricarda Glaum and Margit Wiedmann-Al-Ahmad
28. Isolation and Culture of Skeletal Muscle Myofibers as a Means to Analyze Satellite Cells Paul Keire, Andrew Shearer, Gabi Shefer, and Zipora Yablonka-Reuveni

29. Hepatic Differentiation of Embryonic Stem Cells by Murine Fetal Liver Mesenchymal Cells Takamichi Ishii, Kentaro Yasuchika, and Iwao Ikai

30. Methods to Culture, Differentiate, and Characterize Neural Stem Cells from the Adult and Embryonic Mouse Central Nervous System Sharon A. Louis, Carmen K.H. Mak, and Brent A. Reynolds

31. Feeder-Independent Culture Systems for Human Pluripotent Stem Cells
Jennifer Moody

32. Formation of Embryoid Bodies from Human Pluripotent Stem Cells Using AggreWell™ Plates Jennifer Antonchuk

33. Techniques in Embryoid Body Formation from Human Pluripotent Stem Cells
Nirupama K. Shevde and Amber A. Mael

Aysegul V. Ergen, Mira Jeong, Kuanyin K. Lin, Grant A. Challen, and Margaret A. Goodell

11. Stem Cell Identification by DyeCycle Violet Side Population Analysis William G. Telford

12. Isolation and Characterization of Cancer Stem Cells In Vitro Craig Gedye and Laurie Ailles

13. Ex Vivo Differentiation of Cord Blood Stem Cells into Megakaryocytes and Platelets Nicolas Pineault, Amélie Robert, Valérie Cortin, and Lucie Boyer

14. Generation and Characterization of Murine Alternatively Activated Macrophages Shelley B. Weisser, Keith W. McLarren, Etsushi Kuroda, and Laura M. Sly
15. Human Long Term Culture Initiating Cell (LTC-IC) Assay Min Liu, Cindy Miller, and Connie Eaves

16. Long Term Culture Initiating Cell (LTC-IC) Assay for Mouse Cells Stefan Wohrer, Cindy L. Miller, and Connie J Eaves

17. Colony Forming Cell Assays for Human Hematopoietic Progenitor Cells Bert Wognum, Ning Yuan, Becky Lai, and Cindy L. Miller

18. Studying Leukocyte Recruitment Under Flow Conditions Sean A. Parsons, Christophe Jurzinsky, Susan L. Cuvelier, and Kamala D. Patel

19. Generation and Establishment of Murine Adherent Cell Lines Rouzanna Istvanffy and Robert A.J. Oostendorp

20. Isolation, Enumeration, and Expansion of Human Mesenchymal Stem Cells in Culture Ravenska Wagey and Brenton Short

21. Isolation and Culture of Mesenchymal Stem Cells from Mouse Compact Bone Brenton Short and Ravenska Wagey

22. Generation of a Pool of Human Platelet Lysate (pHPL) and Efficient Use in Cell Culture Katharina Schallmoser and Dirk Strunk

23. In Vitro Methods to Culture Primary Human Breast Epithelial Cells Afshin Raouf and Yu Jia Sun

24. Human Prostate Epithelial Cell Cultures Johng S. Rhim

25. Enzymatic Dissociation, Flow Cytometric Analysis, and Culture of Normal Mouse Mammary Tissue Michael Prater, Mona Shehata, Christine J. Watson, and John Stingl

26. Isolation and Characterization of Human Hair Follicle Epithelial Cells Keita Inoue and Kotaro Yoshimura

27. Co-Cultivation of Human Oral Keratinocytes and Human Osteoblast-Like Cells Ricarda Glaum and Margit Wiedmann-Al-Ahmad
28. Isolation and Culture of Skeletal Muscle Myofibers as a Means to Analyze Satellite Cells Paul Keire, Andrew Shearer, Gabi Shefer, and Zipora Yablonka-Reuveni

29. Hepatic Differentiation of Embryonic Stem Cells by Murine Fetal Liver Mesenchymal Cells Takamichi Ishii, Kentaro Yasuchika, and Iwao Ikai

30. Methods to Culture, Differentiate, and Characterize Neural Stem Cells from the Adult and Embryonic Mouse Central Nervous System Sharon A. Louis, Carmen K.H. Mak, and Brent A. Reynolds

31. Feeder-Independent Culture Systems for Human Pluripotent Stem Cells
Jennifer Moody

32. Formation of Embryoid Bodies from Human Pluripotent Stem Cells Using AggreWell™ Plates Jennifer Antonchuk

33. Techniques in Embryoid Body Formation from Human Pluripotent Stem Cells
Nirupama K. Shevde and Amber A. Mael

William G. Telford

12. Isolation and Characterization of Cancer Stem Cells In Vitro Craig Gedye and Laurie Ailles

13. Ex Vivo Differentiation of Cord Blood Stem Cells into Megakaryocytes and Platelets Nicolas Pineault, Amélie Robert, Valérie Cortin, and Lucie Boyer

14. Generation and Characterization of Murine Alternatively Activated Macrophages Shelley B. Weisser, Keith W. McLarren, Etsushi Kuroda, and Laura M. Sly
15. Human Long Term Culture Initiating Cell (LTC-IC) Assay Min Liu, Cindy Miller, and Connie Eaves

16. Long Term Culture Initiating Cell (LTC-IC) Assay for Mouse Cells Stefan Wohrer, Cindy L. Miller, and Connie J Eaves

17. Colony Forming Cell Assays for Human Hematopoietic Progenitor Cells Bert Wognum, Ning Yuan, Becky Lai, and Cindy L. Miller

18. Studying Leukocyte Recruitment Under Flow Conditions Sean A. Parsons, Christophe Jurzinsky, Susan L. Cuvelier, and Kamala D. Patel

19. Generation and Establishment of Murine Adherent Cell Lines Rouzanna Istvanffy and Robert A.J. Oostendorp

20. Isolation, Enumeration, and Expansion of Human Mesenchymal Stem Cells in Culture Ravenska Wagey and Brenton Short

21. Isolation and Culture of Mesenchymal Stem Cells from Mouse Compact Bone Brenton Short and Ravenska Wagey

22. Generation of a Pool of Human Platelet Lysate (pHPL) and Efficient Use in Cell Culture Katharina Schallmoser and Dirk Strunk

23. In Vitro Methods to Culture Primary Human Breast Epithelial Cells Afshin Raouf and Yu Jia Sun

24. Human Prostate Epithelial Cell Cultures Johng S. Rhim

25. Enzymatic Dissociation, Flow Cytometric Analysis, and Culture of Normal Mouse Mammary Tissue Michael Prater, Mona Shehata, Christine J. Watson, and John Stingl

26. Isolation and Characterization of Human Hair Follicle Epithelial Cells Keita Inoue and Kotaro Yoshimura

27. Co-Cultivation of Human Oral Keratinocytes and Human Osteoblast-Like Cells Ricarda Glaum and Margit Wiedmann-Al-Ahmad
28. Isolation and Culture of Skeletal Muscle Myofibers as a Means to Analyze Satellite Cells Paul Keire, Andrew Shearer, Gabi Shefer, and Zipora Yablonka-Reuveni

29. Hepatic Differentiation of Embryonic Stem Cells by Murine Fetal Liver Mesenchymal Cells Takamichi Ishii, Kentaro Yasuchika, and Iwao Ikai

30. Methods to Culture, Differentiate, and Characterize Neural Stem Cells from the Adult and Embryonic Mouse Central Nervous System Sharon A. Louis, Carmen K.H. Mak, and Brent A. Reynolds

31. Feeder-Independent Culture Systems for Human Pluripotent Stem Cells
Jennifer Moody

32. Formation of Embryoid Bodies from Human Pluripotent Stem Cells Using AggreWell™ Plates Jennifer Antonchuk

33. Techniques in Embryoid Body Formation from Human Pluripotent Stem Cells
Nirupama K. Shevde and Amber A. Mael

Craig Gedye and Laurie Ailles

13. Ex Vivo Differentiation of Cord Blood Stem Cells into Megakaryocytes and Platelets Nicolas Pineault, Amélie Robert, Valérie Cortin, and Lucie Boyer

14. Generation and Characterization of Murine Alternatively Activated Macrophages Shelley B. Weisser, Keith W. McLarren, Etsushi Kuroda, and Laura M. Sly
15. Human Long Term Culture Initiating Cell (LTC-IC) Assay Min Liu, Cindy Miller, and Connie Eaves

16. Long Term Culture Initiating Cell (LTC-IC) Assay for Mouse Cells Stefan Wohrer, Cindy L. Miller, and Connie J Eaves

17. Colony Forming Cell Assays for Human Hematopoietic Progenitor Cells Bert Wognum, Ning Yuan, Becky Lai, and Cindy L. Miller

18. Studying Leukocyte Recruitment Under Flow Conditions Sean A. Parsons, Christophe Jurzinsky, Susan L. Cuvelier, and Kamala D. Patel

19. Generation and Establishment of Murine Adherent Cell Lines Rouzanna Istvanffy and Robert A.J. Oostendorp

20. Isolation, Enumeration, and Expansion of Human Mesenchymal Stem Cells in Culture Ravenska Wagey and Brenton Short

21. Isolation and Culture of Mesenchymal Stem Cells from Mouse Compact Bone Brenton Short and Ravenska Wagey

22. Generation of a Pool of Human Platelet Lysate (pHPL) and Efficient Use in Cell Culture Katharina Schallmoser and Dirk Strunk

23. In Vitro Methods to Culture Primary Human Breast Epithelial Cells Afshin Raouf and Yu Jia Sun

24. Human Prostate Epithelial Cell Cultures Johng S. Rhim

25. Enzymatic Dissociation, Flow Cytometric Analysis, and Culture of Normal Mouse Mammary Tissue Michael Prater, Mona Shehata, Christine J. Watson, and John Stingl

26. Isolation and Characterization of Human Hair Follicle Epithelial Cells Keita Inoue and Kotaro Yoshimura

27. Co-Cultivation of Human Oral Keratinocytes and Human Osteoblast-Like Cells Ricarda Glaum and Margit Wiedmann-Al-Ahmad
28. Isolation and Culture of Skeletal Muscle Myofibers as a Means to Analyze Satellite Cells Paul Keire, Andrew Shearer, Gabi Shefer, and Zipora Yablonka-Reuveni

29. Hepatic Differentiation of Embryonic Stem Cells by Murine Fetal Liver Mesenchymal Cells Takamichi Ishii, Kentaro Yasuchika, and Iwao Ikai

30. Methods to Culture, Differentiate, and Characterize Neural Stem Cells from the Adult and Embryonic Mouse Central Nervous System Sharon A. Louis, Carmen K.H. Mak, and Brent A. Reynolds

31. Feeder-Independent Culture Systems for Human Pluripotent Stem Cells
Jennifer Moody

32. Formation of Embryoid Bodies from Human Pluripotent Stem Cells Using AggreWell™ Plates Jennifer Antonchuk

33. Techniques in Embryoid Body Formation from Human Pluripotent Stem Cells
Nirupama K. Shevde and Amber A. Mael

Nicolas Pineault, Amélie Robert, Valérie Cortin, and Lucie Boyer

14. Generation and Characterization of Murine Alternatively Activated Macrophages Shelley B. Weisser, Keith W. McLarren, Etsushi Kuroda, and Laura M. Sly
15. Human Long Term Culture Initiating Cell (LTC-IC) Assay Min Liu, Cindy Miller, and Connie Eaves

16. Long Term Culture Initiating Cell (LTC-IC) Assay for Mouse Cells Stefan Wohrer, Cindy L. Miller, and Connie J Eaves

17. Colony Forming Cell Assays for Human Hematopoietic Progenitor Cells Bert Wognum, Ning Yuan, Becky Lai, and Cindy L. Miller

18. Studying Leukocyte Recruitment Under Flow Conditions Sean A. Parsons, Christophe Jurzinsky, Susan L. Cuvelier, and Kamala D. Patel

19. Generation and Establishment of Murine Adherent Cell Lines Rouzanna Istvanffy and Robert A.J. Oostendorp

20. Isolation, Enumeration, and Expansion of Human Mesenchymal Stem Cells in Culture Ravenska Wagey and Brenton Short

21. Isolation and Culture of Mesenchymal Stem Cells from Mouse Compact Bone Brenton Short and Ravenska Wagey

22. Generation of a Pool of Human Platelet Lysate (pHPL) and Efficient Use in Cell Culture Katharina Schallmoser and Dirk Strunk

23. In Vitro Methods to Culture Primary Human Breast Epithelial Cells Afshin Raouf and Yu Jia Sun

24. Human Prostate Epithelial Cell Cultures Johng S. Rhim

25. Enzymatic Dissociation, Flow Cytometric Analysis, and Culture of Normal Mouse Mammary Tissue Michael Prater, Mona Shehata, Christine J. Watson, and John Stingl

26. Isolation and Characterization of Human Hair Follicle Epithelial Cells Keita Inoue and Kotaro Yoshimura

27. Co-Cultivation of Human Oral Keratinocytes and Human Osteoblast-Like Cells Ricarda Glaum and Margit Wiedmann-Al-Ahmad
28. Isolation and Culture of Skeletal Muscle Myofibers as a Means to Analyze Satellite Cells Paul Keire, Andrew Shearer, Gabi Shefer, and Zipora Yablonka-Reuveni

29. Hepatic Differentiation of Embryonic Stem Cells by Murine Fetal Liver Mesenchymal Cells Takamichi Ishii, Kentaro Yasuchika, and Iwao Ikai

30. Methods to Culture, Differentiate, and Characterize Neural Stem Cells from the Adult and Embryonic Mouse Central Nervous System Sharon A. Louis, Carmen K.H. Mak, and Brent A. Reynolds

31. Feeder-Independent Culture Systems for Human Pluripotent Stem Cells
Jennifer Moody

32. Formation of Embryoid Bodies from Human Pluripotent Stem Cells Using AggreWell™ Plates Jennifer Antonchuk

33. Techniques in Embryoid Body Formation from Human Pluripotent Stem Cells
Nirupama K. Shevde and Amber A. Mael

Shelley B. Weisser, Keith W. McLarren, Etsushi Kuroda, and Laura M. Sly
15. Human Long Term Culture Initiating Cell (LTC-IC) Assay Min Liu, Cindy Miller, and Connie Eaves

16. Long Term Culture Initiating Cell (LTC-IC) Assay for Mouse Cells Stefan Wohrer, Cindy L. Miller, and Connie J Eaves

17. Colony Forming Cell Assays for Human Hematopoietic Progenitor Cells Bert Wognum, Ning Yuan, Becky Lai, and Cindy L. Miller

18. Studying Leukocyte Recruitment Under Flow Conditions Sean A. Parsons, Christophe Jurzinsky, Susan L. Cuvelier, and Kamala D. Patel

19. Generation and Establishment of Murine Adherent Cell Lines Rouzanna Istvanffy and Robert A.J. Oostendorp

20. Isolation, Enumeration, and Expansion of Human Mesenchymal Stem Cells in Culture Ravenska Wagey and Brenton Short

21. Isolation and Culture of Mesenchymal Stem Cells from Mouse Compact Bone Brenton Short and Ravenska Wagey

22. Generation of a Pool of Human Platelet Lysate (pHPL) and Efficient Use in Cell Culture Katharina Schallmoser and Dirk Strunk

23. In Vitro Methods to Culture Primary Human Breast Epithelial Cells Afshin Raouf and Yu Jia Sun

24. Human Prostate Epithelial Cell Cultures Johng S. Rhim

25. Enzymatic Dissociation, Flow Cytometric Analysis, and Culture of Normal Mouse Mammary Tis