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Preface v
Ackowledgments vii
Contributors xviii
Color Plate xvii
Contents for Volume 2 xix
Part I Synovial Joint Morphology, Histopathology, and Immunohistochemistry
1 Imaging Inflamed Synovial Joints Ai Lyn Tan Helen I. Keen Paul Emery Dennis McGonagle 3
2 Arthroscopy as a Research Tool: A Review Richard J. Reece 27
3 Immunohistochemistry of the Inflamed Synovium Martina Gogarty Oliver FitzGerald 47
4 In Situ Hybridization of Synovial Tissue Stefan Kuchen Christian A. Seemayer Michel Neidhart Renate E. Gay Steffen Gay 65
5 Subtractive Hybridization Jo&omlet;rg H. W. Distler Oliver Distler Michel Neidhart Steffen Gay 77
6 Laser Capture as a Tool for Analysis of Gene Expression in Inflamed Synovium Ulf Mu&omlet;ller-Ladner Martin Judex Elena Neumann Steffen Gay 91
7 Preparation of Mononuclear Cells from Synovial Tissue Jonathan T. Beech Fionula M. Brennan 105
8 Quantitative Image Analysis of Synovial Tissue Pascal O. van der Hall Maarten C. Kraan Paul Peter Tak 121
Part II Cartilage Matrix and Bone Biology
9 Cartilage Histomorphometry Ernst B. Hunziker 147
10 Image Analysis of Aggrecan Degradation in Articular Cartilage with Formalin-Fixed Samples Barbara Osborn Yun Bai Anna H. K. Plaas John D. Sandy 167
11 In Situ Detection of Cell Death in Articular Cartilage Samantha N. Redman Ilyas M. Khan Simon R. Tew Charles W. Archer 183
12 Measurement of Glycosaminoglycan Release from Cartilage Explants John S. Mort Peter J. Roughley 201
13 Assessment of Collagenase Activity in Cartilage Tim E. Cawston Tanya G. Morgan 211
14 Assessment of Gelatinase Expression and Activity in Articular Cartilage Rosalind M. Hembry Susan J. Atkinson Gillian Murphy 227
15Analysis of MT1-MMP Activity in Cells Richard D. Evans Yoshifumi Itoh 239
16 Analysis of TIMP Expression and Activity Linda Troeberg Hideaki Nagase 251
17 Bone Histomorphology in Arthritis Models Georg Schett Birgit Tuerk 269
18 Generation of Osteoclasts In Vitro, and Assay of Osteoclast Activity Naoyuki Takahashi Nobuyuki Udagawa Yasuhiro Kobayashi Tatsuo Suda 285
Part III Cell Trafficking, Migration, and Invasion
19 Isolation and Analysis of Large and Small Vessel Endothelial Cells Justin C. Mason Elaine A. Lidington Helen Yarwood 305
20 Analysis of Flow-Based Adhesion In Vitro Oliver Florey Dorian O. Haskard 323
21 Analysis of Leukocyte Recruitment in Synovial Microcirculation by Intravital Microscopy Gabriela Constantin 333
22 Angiogenesis in Arthritis: Methodological and Analytical Details Ursula Fearon Douglas J. Veale 343
23 Analysis of Inflammatory Leukocyte and Endothelial Chemotactic Activity Zolta´n Szekanecz Alisa Koch 359
24 Acquisition, Culture, and Phenotyping of Synovial Fibroblasts Sanna Rosengren David L. Boyle Gary S. Firestein 365
25 Genotyping of Synovial Fibroblasts: cDNA Array in Combination with RAP-PCR in Arthritis Elena Neumann Martin Judex Steffen Gay Ulf Mu&omlet;ller-Ladner 377
26 Gene Transfer to Synovial Fibroblast: Methods and Evaluation in the SCID Mouse Model Ingmar Meinecke Edita Rutkauskaite Antje Cinski Ulf Mu&omlet;ller-Ladner Steffen Gay Thomas Pap 393
27 In Vitro Matrigel Fibroblast Invasion Assay Tanja C.A. Tolboom Tom W.J. Huizinga 413
28 Culture and Analysis of Circulating Fibrocytes Timothy E. Quan Richard Bucala 423
Index 435
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Michael Self
reviewed Arthritis Research: Methods and Protocols, Vol. 1 on April 28, 2008 Arthritis Research
FAAAASCINATING. Honestly, I kept pestering Kent, "Listen to this..." and then reading a paragraph or two. It's about the science of how the brain and body connect. We have "body maps" in our brain, that tell us exactly where our body parts are, and how they are doing. (This is why we can touch our nose with our eyes closed.) It doesn't sound interesting, until you read what this means, in practical applications. And the stuff about how our homonulcus (body maps) go awry is just astonishing.
Body mapping accounts for why we can ride a horse, why we duck a little when we drive our cars into a garage with low clearance, why we feel afraid in scary movies, and also things like autism (mirror neuron defects).
Here's an example: if you took a perfectly healthy kitten, and then carried it around with you wherever you went, exposing it to many sights and sounds, but not allowing it to interact with it's environment, that kitten would be blind. It would still "see" colors and shapes and everything, but it's brain would have no context for interpreting that input, so the cat would be just as blind as a cat with no eyes. Bizarre, huh? Here's another: the women in Mali think it's important for their children to have straight legs so several times a day, from birth onwards, they massage and pull on their infants' legs, pulling them straight. Guess the average age of walking? EIGHT MONTHS. Because those kids' body maps of their legs got so much stimulation from the environment as they were developing. Contrast that with kids in orphanages in the Eastern Block countries who are left alone in their cribs for days on end. Those kids are unable to stand on one foot without toppling over. Why? Because their leg body maps are so woefully undeveloped because there was no opportunity for interaction with their environment.
Anyhow, it's really an interesting read, runs a gamut of really diverse topics, all having to do with our body maps, and it's not very long (about 200 pages.) I really recommend it.
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