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Chromosome Techniques: Manual Book

Chromosome Techniques: Manual
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  • Chromosome Techniques: Manual
  • Written by author Arun K. Sharma, Archana Sharma
  • Published by CRC Press, 1994/06/30
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Preface
Introduction
Ch. 1 Pre-Treatment, Hypotonic Treatment, Fixation and Staining 1
Ch. 2 Microscopy, Microspectrophotometry, Flow Cytometry, Image Analysis, Confocal Microscopy 33
Ch. 3 Study of Banding Patterns of Chromosomes 49
Ch. 4 Autoradiography - Light, Fibre and High Resolution 82
Ch. 5 Electron Microscopy 94
Ch. 6 Study of Chromosomes From Tissue and Protoplast Culture, Cell Fusion and Gene Transfer in Plants 115
Ch. 7 Chromosome Analysis Following Short- and Long-Term Cultures in Mammalian and Human Systems, From Normal and Malignant Tissues 130
Ch. 8 Somatic Cell Fusion in Animals 161
Ch. 9 Effects of External Agents and Monitoring for Environmental Toxicants 168
Ch. 10 Isolation and Extraction of Nuclei, Chromosomes and Components 197
Ch. 11 Micrurgy 221
Ch. 12 Identification of Chromosome Segments and DNA Sequences by In Situ Molecular Hybridization 227
Ch. 13 Special Molecular Techniques Necessary for Chromosome Analysis 274
Ch. 14 Representative Schedules for Direct Observation of Chromosomes from Plants and Animals In Vivo and from Special Materials 297
References 333
Index 365


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