Gene Transfer to Plants : Springer Lab Manual Book

Pt. I	Agrobacterium tumefaciens-Mediated Gene Transfer	1
	Introduction I Agrobacterium tumefaciens, a Natural Vector System	3
1	Transformation of Arabidopsis thaliana Using Root Explants	5
2	Seed Transformation in Arabidopsis thaliana	11
3	In Planta Agrobacterium-Mediated Transformation of Adult Arabidopsis thaliana Plants by Vacuum-Infiltration	19
4	Agrobacterium-Mediated Transformation of Potato (Solanum tuberosum)	24
5	Agrobacterium-Mediated Transformation of Rapeseed (Brassica napus)	30
Pt. II	Agrobacterium rhizogenes-Mediated Gene Transfer	39
	Introduction II Agrobacterium rhizogenes, a Natural Transformation System	41
6	Agrobacterium rhizogenes-Mediated Transformation: Transformed Roots to Transformed Plants	45
Pt. III	Direct Gene Transfer to Protoplasts	53
	Introduction III Direct Gene Transfer to Protoplasts	55
7	Polyethylene Glycol-Mediated Direct Gene Transfer to Tobacco Protoplasts and Regeneration of Transgenic Plants	58
8	Polyethylene Glycol-Mediated Direct Gene Transfer to Indica Rice Protoplasts and Regeneration of Transgenic Plants	66
9	Plastid Transformation by Polyethylene Glycol Treatment of Protoplasts and Regeneration of Transplastomic Tobacco Plants	75
10	Electroporation-Mediated Gene Transfer to Protoplasts and Regeneration of Transgenic Plants	83
11	Electroporation Mediated Gene Transfer to Japonica Rice Protoplasts and Regeneration of Transgenic Plants	93
12	Regeneration of Transgenic Maize Plants from Embryogenic Protoplasts After Polyethylene Glycol-Mediated DNA Uptake	99
13	Gene Transfer to Protoplasts: Transient Gene Expression Analysis	106
Pt. IV	Biolistic Plant Transformation	113
	Introduction IV The Biolistic Transformation System	115
14	Transient Gene Expression Studies with the Biolistic System	118
15	Maize Transformation by Microprojectile Bombardment of Immature Embryos	127
16	Biolistic Transformation of Microspore-Derived and Immature Zygotic Embryos and Regeneration of Fertile Transgenic Barley Plants	139
17	Transformation of Soybean (Glycine max) Through Electric Discharge Particle Acceleration	147
18	Ballistic Microtargeting of Visible Marker Genes to the Shoot Meristem of Wheat	152
19	Stable Transformation of Wheat by Microprojectile Bombardment	157
20	Biolistic-Based Analysis of Chloroplast Gene Expression	162
Pt. V	Microinjection and Fiber-Mediated Transformation	171
	Introduction V Microinjection into Plant Cells: Methodology and Applications	173
21	Microinjection into Tobacco Protoplasts and Regeneration of Transgenic Plants	176
22	Silicon Carbide Whisker-Mediated Transformation: Regeneration of Transgenic Maize Plants	186
Pt. VI	Tissue Electroporation	193
	Introduction VI Transformation by Tissue-Electroporation	195
23	Transient Expression Following Electroporation into Immature Zygotic Embryos of Wheat	196
24	Transgenic Indica Rice Plants by Electroporation of Seed Embryo Cells	201
Pt. VII	Analysis of Transgenic Plants	209
	Introduction VII Analysis of Transgenic Plants	211
25	Standard Molecular Techniques for the Analysis of Transgenic Plants
26	In Situ Hybridization on Plant Metaphase Chromosomes	264
27	Localisation of RNA Transcripts in Plant Tissue by in Situ Hybridisation	273
28	In Situ Immunolocalisation of Transgene Products in Transgenic Plants	286
Pt. VIII	Establishment and Maintenance of Embryogenic Cultures	291
	Introduction VIII Embryogenic Cultures: a Source of Target Cells for Gene Transfer	293
29	Establishment of and Plant Regeneration from Embryogenic Cell Suspensions and Their Protoplasts in Forage Grasses	295
Pt. IX	Genetic Markers and Expression Signals	305
	Introduction IX Genetic Markers and Expression Signals	307
30	Expression Signals and Vectors	311
31	Selectable Marker and Reporter Genes	325
Pt. X	Guidelines and Biosafety Considerations	337
	Introduction X Gene Technology with Plants and the Public	339
32	Release of Transgenic Plants: Regulations and Developments	340
33	Risk Assessment	350