Proteomics in Practice: A Laboratory Manual of Proteome Analysis Book

Proteomics in Practice: A Laboratory Manual of Proteome Analysis, , Proteomics in Practice: A Laboratory Manual of Proteome Analysis

3 out of 5 stars based on 2 reviews

Proteomics in Practice: A Laboratory Manual of Proteome Analysis
Written by author Reiner Westermeier
Published by Wiley, John & Sons, Incorporated, August 2002
A combined review, manual and reference for the successful analysis of proteins using the classical approach of 2-D gel electrophoresis, mass spectrometry and related sequence database inquiries.The first section, written in a textbook style, introduce

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Preface
Foreword
Abbreviations, symbols, units
Glossary of terms
Pt. I Proteomics Technology 1
Introduction 1
Expression proteomics 11
Two-dimensional Electrophoresis 11
Spot handling 97
Mass spectrometry 108
Protein identification by database searching 135
Methods of proteome analysis 154
Pt. II Course Manual 161
Step 1 Sample preparation 169
Step 2 Isoelectric focusing 187
Step 3 SDS Polyacrylamide Gel Electrophoresis 199
Step 4 Staining of the gels 233
Step 5 Scanning of gels and image analysis 239
Step 6 Fluorescence difference gel electrophoresis 249
Step 7 Spot excision 255
Step 8 Sample destaining 259
Step 9 In-gel digestion 261
Step 10 Microscale desalting and concentration of sample 263
Step 11 Chemical derivatisation of the peptide digest 265
Step 12 MS analysis 269
Step 13 Calibration of the MALDI-ToF MS 273
Step 14 Preparing for a database search 277
Step 15 PMF database search unsuccessful 281
A Trouble shooting 283
References 295
Index 311

Title: Proteomics in Practice: A Laboratory Manual of Proteome Analysis

Manufacturer:

Wiley

Item Number: 9783527303540

Publication Date: August 2002

Product Description: Full Name: Proteomics in Practice: A Laboratory Manual of Proteome Analysis; Short Name:Proteomics in Practice

Universal Product Code (UPC): 9783527303540

WonderClub Stock Keeping Unit (WSKU): 9783527303540

Rating: 3/5 based on 2 Reviews

Image Location: https://wonderclub.com/images/covers/35/40/9783527303540.jpg

Category: Media >> Books

Weight: 0.200 kg (0.44 lbs)

Width: 0.000 cm (0.00 inches)

Heigh : 0.000 cm (0.00 inches)

Depth: 0.000 cm (0.00 inches)

Date Added: August 25, 2020, Added By: Ross

Date Last Edited: August 25, 2020, Edited By: Ross

Price	Condition	Delivery	Seller	Action
$130.00	Digital	Arrives between May 12 - Jun 11 Login to your account for a direct download. Some files are emailed from Dropbox. Continent $0.00 - World $0.00	WonderClub (9296 total ratings)

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Carol Todesco

reviewed Proteomics in Practice: A Laboratory Manual of Proteome Analysis on September 26, 2015

Proteomics in Practice

I agree - this is a wonderful, comprehensive book.
Also, it has very nice pictures - which are a must.

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