Gene Therapy of Cancer: Methods and Protocols Book

	Preface
	Contributors
Pt. I	Experimental Approaches in Cancer Gene Therapy
A	Immunotherapy/Tumor Vaccination
1	Immunizing Potential of Cytokine-Transduced Tumor Cells	3
2	Particle-Mediated Gene Transfer into Dendritic Cells: A Novel Strategy for the Induction of Immune Responses against Tumor Antigens	27
3	Cancer Gene Therapy with Heat Shock Protein-65 Gene	49
4	Recombinant Vaccinia Virus MVA for Generation and Analysis of T Cell Responses Against Tumor Associated Antigens	57
B	Suicide Gene Therapy
5	Selection of Cytochrome P450 Genes for Use in Prodrug Activation-Based Cancer Gene Therapy	77
6	Construction of P450-Expressing Tumor Cell Lines Using Retroviruses	85
7	In Vitro Methods for Evaluation of P450-Based Anticancer Gene Therapy	95
8	Tumor Models for Evaluation of P450 Gene Therapy In Vivo	107
C	Anti-Oncogene and Suppressor Gene Therapy
9	Intracellular Single-Chain Antibodies for Gene Therapy	121
10	Combined Adenoviral Transfer of Tumor Suppressor and Cell-Cycle Genes for Tumor-Cell Apoptosis	151
D	Antisense Gene Therapy
11	Inhibition of Cell Growth by Antisense Oligonucleotides Targeting the Growth-Related Protein Kinase c-raf	167
12	IGF-1 Antisense Strategies for Cancer Treatment	189
E	Ribozyme Gene Therapy
13	Anti-MDR1 Ribozyme Gene Therapy	207
14	Anti-c-erbB2 Ribozyme for Gene Therapy of Breast Cancer	247
15	Anti-K-ras Ribozyme Adenoviral Vector for Gene Therapy of Non-Small Cell Lung Cancer	261
F	Delivery Systems and Tumor Targeting
16	Green Fluorescent Protein Retroviral Vector: Generation of High-Titer Producer Cells and Virus Supernatant	275
17	HSV-1 Vectors for Gene Therapy of Experimental CNS Tumors	287
18	Intratumoral Injection of Naked DNA	313
19	Cationic Liposome Gene Transfer	323
20	In Vivo Particle-Mediated Gene Transfer for Cancer Therapy	331
21	Gene Targeting to Hepatomas (AFP)	345
22	Adenovirus-Mediated Targeted Gene Therapy for Breast Cancer and for Purging Hematopoietic Stem-Cell Sources	361
23	Chemotherapy-Inducible Vector for Gene Therapy of Cancer	371
G	Alternative Approaches in Cancer Gene Therapy
24	Oncolytic Adenoviral Vectors	395
25	Genetically Modified Clostridium for Gene Therapy of Tumors	413
26	Tumor-Targeted Salmonella: Strain Development and Expression of the HSV-tK Effector Gene	419
Pt. II	Clinical Protocols for Cancer Gene Therapy
A	Immunotherapy/Tumor Vaccination
27	Ex Vivo Cytokine Gene Transfer in Melanomas by Using Particle Bombardment	439
28	Intratumoral Gene Transfer of the HLA-B7 Gene Into Colon Carcinoma Metastases	453
29	Hybrid Cell Vaccination in Patients With Metastatic Melanoma	469
B	Suicide Gene Therapy
30	Retroviral Transfer of the Herpes Simplex Virus-Thymidine Kinase (HSV-tK) Gene for the Treatment of Cancer	479
31	Gene Therapy for Treatment of Brain Tumors (HSV-tK In Vivo Gene Transfer): A Case Study	499
32	Gene Therapy of Glioblastoma Multiforme with a Bicistronic Retroviral Vector Expressing Human IL-2 and HSV-tk	511
33	Intratumoral Gene Transfer of the Cytosine Deaminase Gene for the Treatment of Breast Cancer	523
C	Anti-Oncogene and Suppressor Gene Therapy
34	Adenovirus-Mediated Wild-Type p53 Gene Transfer into Head and Neck Cancers	537
35	Direct DNA Injection (p53) into HCC Tumors	545
36	A Phase II Trial of Intratumoral Injection with a Selectively Replicating Adenovirus (ONYX-015) in Patients with Recurrent, Refractory Squamous Cell Carcinoma of the Head and Neck	559
D	Antisense Gene Therapy
37	c-myb Antisense Oligonucleotide Therapeutics for Hematologic Malignancies	577
38	Ovarian Cancer Gene Therapy with BRCA1 - An Overview	593
39	Methods for Chemoprotection and Chemosensitization: MDR-1 For Chemoprotection Using Retroviruses to Modify Hematopoietic Cells and Cytosine Deaminase for Chemosensitization Using Adenoviral Vectors to Modify Epithelian Neoplastic Cells	609
	Index	617