Flow Cytometry Protocols Book

	Preface
	Contributors
1	Flow cytometry : an introduction	1
2	Multiparameter flow cytometry of bacteria	33
3	Multiparameter data acquisition and analysis of leukocytes	45
4	Flow cytometric analysis of kinase signaling cascades	67
5	Cytokine flow cytometry	95
6	Use of cell-tracking dyes to determine proliferation precursor frequencies of antigen-specific T cells	109
7	Assessment of lymphocyte-mediated cytotoxicity using flow cytometry	125
8	Multiparametric analysis of apoptosis by flow and image cytometry	141
9	Detection and enrichment of haematopoietic stem cells by side population phenotype	161
10	Hematopoietic stem cell characterization by Hoechst 33342 and Rhodamine 123 staining	181
11	Phenotypic and functional analyses of CD34[superscript NEG] haematopoietic precursors from mobilized peripheral blood	201
12	Multiparameter flow cytometry of fluorescent protein reporters	219
13	Analysis of fluorescent protein expressing cells by flow cytometry	239
14	Integrative flow cytometric and microarray approaches for use in transcriptional profiling	259
15	Flow cytometric analysis of fluorescence resonance energy transfer : a tool for high-throughput screening of molecular interactions in living cells	281
16	Design of a fluorescence-activated cell sorting-based mammalian protein-protein interaction trap	293
17	Flow cytometric screening of yeast surface display libraries	311
18	Fluorescence resonance energy transfer-based HIV-1 virion fusion assay	333
19	Cell-cycle analysis of asynchronous populations	345
20	Solid tumor DNA content analysis	355
21	Concurrent flow cytometric analysis of DNA and RNA	371
22	Telomere length measurement by fluorescence in situ hybridization and flow cytometry	385
23	Small lasers in flow cytometry	399
24	Viable infections cell sorting in a BSL-3 facility	419
	Index	425