Colorectal Cancer: Methods and Protocols Book

Microdissection of Histological Sections: Manual and Laser Capture Microdissection Techniques

Christopher A. Moskaluk

Isolation of a Purified Epithelial Cell Population from Human Colon

James K. Roche

Xenografting Human Colon Cancers

Jeffrey C. Harper, Reid B. Adams, and Steven M. Powell

Comparative Genomic Hybridization Technique

Wa'el El-Rifai and Sakari Knuutila

Fluorescence In Situ Hybridization: Application in Cancer Research and Clinical Diagnostics

Svetlana D. Pack and Zhengping Zhuang

Microsatellite Analysis to Assess Chromosome 18q Status in Colorectal Cancer

Jin Jen

The SURF Technique: Selective Genetic Analysis of Microscopic Tissue Heterogeneity

Darryl Shibata

Microsatellite Instability Testing

Yann R. Parc and Kevin C. Halling

Immunohishemical Analysis for hMLH1 and hMSH2 Expression in Colorectal Cancer

Kevin C. Halling and Patrick C. Roche

Mutation Detection in Colorectal Cancers: Direct Sequencing of DNA Mismatch Repair Genes

Julie M. Cunningham, David J. Tester, and Stephen N. Thibodeau

Mutation Detection in the TGF-b Receptors

William M. Grady, Lois L. Myeroff, Hongmei He, and Sanford Markowitz

Direct Analysis for Familial Adenomatous Polyposis Mutations

Steven M. Powell

I1307K Mutation Detection by Allele-Specific PCR in Familial Colorectal Cancer

Perry S. Chan and Steven L. Gersen

Microsatellite Analysis of the Insulin-Like Growth Factor II Receptor in Colorectal Carcinomas

Rhonda F. Souza and Stephen J. Meltzer

Molecular Detection of Smad2/Smad4 Alterations in Colorectal Tumors

Sam Thiagalingam

Direct Sequencing for Juvenile Polyposis Gene SMAD4/DPC4 Mutations

Lauri A. Aaltonen and Stina Roth

Direct Sequencing for Peutz-Jehgers Gene LKB1 (STK11) Mutations

Lauri A. Aaltonen and Egle Avizienyte

Direct Sequencing for Cowden Syndrome Gene PTEN (MMAC1) Mutations

Lauri A. Aaltonen, Stina Roth, and Charis Eng

Genetic Analysis Using Microarrays

Wa'el El-Rifai and Sakari Knuutila

Cyclooxygenase-2 (COX-2) Protein Expression by Western Blotting

Hossam M. Kandil and Raymond N. DuBois

The Immunohishemical Method

Lisa A. Cerilli and Henry F. Frierson, Jr

Immunohishemical Detection and Quantitation of Cell Surface Receptors for Prostanoids

Rosana Cosme and James K. Roche

Adhesion Signaling Through Integrins

Reid B. Adams and Joshua D. Rovin

Electrophoretic Mobility Shift Assay (EMSA)

Michael F. Smith, Jr. and Sandrine Delbary-Gossart

Transfection Assays for Transformation of Model Colonic Cell Lines

Mark T. Worthington and Roger Qi Luo

Assessment of Intestinal Stem Cell Survival Using the Microcolony Formation Assay

Kimberly S. Tustison, Joanne Yu, and Steven M. Cohn

Gene Transfer into the Colonic Mucosa

Baçak Çoruh and Theresa T. Pizarro

Index