Metastasis Research Protocols Book

1. Basic Immunocyhemistry for Light Microscopy Susan A. Brooks
2. Lectin Hishemistry to Detect Altered Glycosylation in Cells and Tissues Susan A. Brooks & Debbie M.S. Hall
3. Histopathological Assessment of Metastasis Derek E Roskell and Ian D Buley
4. Immunocyhemical Detection and Characterisation of Individual Micrometastatic Tumour Cells Stephan Braun and Klaus Pantel
5. The Polymerase Chain Reaction (PCR)
Hazel M. Welch
6. SDS-PAGE and Western Blotting Techniques Christine Blancher and Rob Mc Cormick
7. 2-DE Based Proteomics for the Analysis of Metastasis Associated Proteins Miriam V Dwek and Diluka Peiris
8. Assessment of Gelatinases (MMP-2 and MMP-9) by Gelatin Zymography Marta Toth, Anjum Sohail, and Rafael Fridman
9. Determination of Cell Specific Receptor Binding using a Combination of Immunohishemistry and in vitro Autoradiography: Relevance to Therapeutic Receptor Targeting in Cancer Michael R. Dashwood and Marilena Loizidou
10. Fluorescence in situ Hybridization (FISH) for Cancer Related Studies Lyndal Kearney and Janet Shipley
11. Using a Quartz Crystal Microbalance Biosensor for the Study of Metastasis Markers on Intact Cells Julien Saint Guirons and Björn Ingemarsson
12. Cell Separations by Flow Cytometry Derek Davies
13. Detection of Putative Cancer Stem Cells of the Side Population Phenotype in Human Tumor Cells Cultures Matthias Christgen, Matthias Ballmaier, Ulrich Lehmann and HansKreipe
14. Genes Involved in the Metastatic Cascade of Medullary Thyroid TumoursCaroline Schreiber, Kirsten Vormbrockand Ulrike Ziebold
15. High-resolution Quantitative Methylation Analysis of microRNA Genes using Pyrosequencing™
Ulrich Lehmann, Cord Albat, Hans Kreipe
16. RNAi Technology to Block the Expression of Molecules Relevant to Metastasis: The Cell Adhesion Molecule CEACAM1 as an Instructive Example Daniel Wicklein

17. Galectin-3-Binding and Metastasis Pratima Nangia-Makker, Vitaly Balan, and Avraham Raz

18. Lectin Array Based Strategies for Identifying Metastasis-associated Changes in Glycosylation Simon Fry, Babak Afrough, Anthony Leathem and Miriam V Dwek