Molecular Biomethods Handbook Book

Molecular Biomethods Handbook 2nd Ed

Contents

PART A: NUCLEIC ACID BIOMETHODS

1 The manipulation of nucleic acids: basic tools and techniques – Gayle Corkill and

Ralph Rapley

2 Restriction Enzymes – Gareth J.S. Jenkins

3 Principles and Medical Applications of the Polymerase Chain Reaction – Birmal D.M.

Theophilus

4 Probe Design, Production, and Applications – Marilena Aquino de Muro

5 Southern Blotting as a Diagnostic Method – Bronwen Harvey and Pirkko Soundy

6 Introduction to Capillary Electrophoresis of DNA - Beatriz Sanchez-Vega

7 Denaturing High-Performance Liquid Chromatography (DHPLC) for nucleic acid

analysis - Kim Hung Leung and Shea Ping Yip

8 Denaturing Gradient Gel Electrophoresis (DGGE) – Jeroen H. Roelfsema and Dorien

J.M. Peters

9 Single Strand Conformation Polymorphism (SSCP) Analysis - Kim Hung Leung and

Shea Ping Yip

10 Randomly amplified polymorphic DNA (RAPD); a useful tool for genomic

characterization of different organisms - Lúcia Maria da Cunha Galvão and Eliane

Lages-Silva

11 Quantification of mRNA using Real Time RT-PCR – the SYBR solution - David

Sugden and Patricia de Winter

12 Quantitative Analysis of DNA Sequences by PCR and Solid Phase Sequencing – Anu

Suomalainen and Ann-Christine Syvanen

13 MAPH (Multiplex Amplifiable Probe Hybridization) - Carolina Sismani, Ludmila

Kousoulidou and Philippos C. Patsalis

14 Gene Expression Profiling - Arnis Druka, Robbie Waugh and Pete Hedley

15 Comparative Genomic Hybridization in Clinical and Medical Research – Peng-Hui

Wang, Yann-Jang Chen and Chi-Hung Lin

16 Subtractive Hybridization – Craig Winstanley

17 Fluorescence in situ Hybridization - Jane Bayani and Jeremy A. Squire

18 Quantitative Trait Locus Mapping to Identify Genes for Complex Traits in Mice -

Jonathan D. Smith

19 cDNA Microarrays – Phillip G. Febbo

20 Mapping Techniques – Simon G. Gregory

21 Single Nucleotide Polymorphisms Overview – Nameeta Shah

22 Bioinformatics Tools for Gene and Protein Sequence Analysis– Bernd H.A. Rehm and

Frank Reinecke

PART B: PROTEIN AND CELL METHODS

23. Protein Electrophoresis - David Sheehan and Siobhan O’Sullivan

24. Protein blotting - Patricia Gravel

25. Capillary electrophoresis of proteins - Mark Strege

26. Autoradiography and fluorography - Bronwen Harvey

27. Mass spectrometry of proteins & peptides - Kenneth G. Standing

28. Post-translational modifications - Christoph Kannicht and Birte Fuchs

29. Protein microarray technology - Charlotte H. Clarke and Eric T. Fung

30. Protein-protein interactions - Hae Ryoun Park, Lisa Montoya Cockrell, Yuhong Du, Andrea Kasinski, Jonathan Havel, Jing Zhao, Francisca Reyes-Turcu, Keith Wilkinson and Haian Fu

31. Glycoprotein analysis - Terry Butters and David C.A. Neville

32. Solid Phase Peptide Synthesis - Mare Cudic and Gregg B. Fields

33. Monoclonal antibodies - Zhong J. Zhang and Maher Albitar

34. Antibody Phage display - Rob Aitken

35. Protein engineering - Thomas Willemsen, Urs B. Hagemann,

Eva M. Jouaux, Sabine Stebel, Jody M. Mason, Kristian M. Müller and Katja M. Arndt

36. Directed protein evolution - Sabine C. Stebel, Annette Gaida, Katja M. Arndt and Kristian M. Müller

37. Enzyme Linked Immunosorbent Assay - John Crowther

38. Epitope mapping - Glenn E. Morris

39. Quantum dots - Charles Hotz

40. Ion exchange chromatography - David Sheehan

41. Size exclusion chromatography - Paul Cutler

42. Hydrophobic interaction chromatography - Paul A. O’Farrell

43. Affinity chromatography - Adam Charlton and Michael Zachariou

44. High Performance Liquid Chromatography

(HPLC) of Proteins and Peptides - Tzong-Hsien Lee and Marie-Isabel Aguilar

45. Amino acid analysis - Ian Davidson + Paula O’Connor

46. Surface plasmon resonance - K. Scott Phillips and Quan Cheng

47. Macromolecular Crystallography - Bernhard Rupp and Kathrine A. Kantardjieff

48. Microchip Devices for Bioanalysis - Anna Kinsella and Shelley D. Minteer

49. Mammalian cell culture - Simon Langdon

50. Plant tissue culture - V.M. Loyola Vargas, C. De-la-Peña, R.M. Galaz-Avalos and F. R. Quiroz-Figueroa

51. Stem cells and Regenerative Medicine - Mohan C. Vemuri and Chellu S. Chetty

52. Cryopreservation : Conservation of Bioresources

at Ultra-Low Temperatures - J.G. Day, K. Harding,

J. Nadarajan and E.E. Benson

53. Magnetic resonance imaging (MRI) - Pottumarthi V. Prasad and Pippa Storey

54. Electron microscopy - John Kuo

55. Confocal microscopy - Guy Cox

56. Laser microdissection - Graeme I. Murray

57. Biomedical uses of Flow cytometry - James L. Weaver and Maryalice Stetler-Stevenson

58. Immunomicroscopy - Constance Oliver

59. In-situ hybridization - Ian A. Darby and Tim D. Hewitson

60. High throughput screening - William P. Janzen