Pyrosequencing Protocols Book

Foreword     v
Preface     vii
Contributors     xi
The History of Pyrosequencing   Pal Nyren     1
Pyrosequencing Applications   Sharon Marsh     15
Web-Based Primer Design Software for Genome-Scale Genotyping by Pyrosequencing   Steven Ringquist   Christopher Pecoraro   Ying Lu   Alexis Styche   William A. Rudert   Panayiotis V. Benos   Massimo Trucco     25
Pyrosequencing: A Simple Method for Accurate Genotyping   Cristi R. King   Tiffany Scott-Horton     39
Universal Primer Applications for Pyrosequencing   Dong-Chuan Guo   Dianna M. Milewicz     57
Allele Quantification and DNA Pooling Methods   Jonathon Wasson     63
Multiplex Pyrosequencing for DNA Variation Analysis   Pritesh Patel   Yih-Horng Shiao   Paolo Fortina     75
Analysis of Gene-Specific DNA Methylation Patterns by Pyrosequencing Technology   Jorg Tost   Ivo Glynne Gut     89
Detecting Mutations That Confer Oxazolidinone Resistance in Gram-Positive Bacteria   Neil Woodford   Sarah E. North   Matthew J. Ellington     103
Pyrosequencing-Based Strategies for ImprovedAllele Typing of Human Leukocyte Antigen Loci   Steven Ringquist   Alexis Styche   William A. Rudert   Massimo Trucco     115
Pyrosequencing of Phage Display Libraries for the Identification of Cell-Specific Targeting Ligands   Ahad A. Rahim     135
Gene Copy Number Detection in Animal Studies   Gerli Pielberg   Leif Andersson     147
Detection of Allelic Imbalance in Gene Expression Using Pyrosequencing   Hua Wang   Steven C. Elbein     157
Pyrosequencing Genotype Storage Techniques   Derek J. Van Booven     177
Index     187