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Preface v
Contributors ix
1 Rat Carotid Artery Balloon Injury Model David A. Tulis 1
2 Histological and Morphometric Analyses for Rat Carotid Balloon Injury Model David A. Tulis 31
3 Plaque Rupture Model in Mice Takeshi Sasaki Kae Nakamura Masafumi Kuzuya 67
4 Immunostaining of Mouse Atherosclerotic Lesions Hong Lu Debra L. Rateri Alan Daugherty 77
5 Surgical Animal Model of Ventricular Hypertrophy Giuseppe Marano Alberto U. Ferrari 95
6 Animal Models of Hypertension Brett M. Mitchell Thomas Wallerath Ulrich Forstermann 105
7 Rat Models of Cardiac Insulin Resistance Sanjoy Ghosh Brian Rodrigues Jun Ren 113
8 The Isolated, Perfused Pseudo-Working Heart Model Gary J. Grover Rajni Singh 145
9 Altering and Analyzing Glucose Metabolism in Perfused Hearts of Transgenic Mice Rajakumar V. Donthi Paul N. Epstein 151
10 Methods in the Evaluation of Cardiovascular Renin Angiotensin Aldosterone Activation and Oxidative Stress Camila Manrique Guido Lastra Javad Habibi Yongzhong Wei E. Matthew Morris Craig S. Stump James R. Sowers 163
11 Detection and Quantification of Apoptosis in the Vasculature Wendy A. Boivin David J. Granville 181
12 Endothelial Progenitor Cell and Mesenchymal Stem Cell Isolation, Characterization, Viral Transduction Keith R. Brunt Sean R. R. Hall Christopher A. Ward Luis G. Melo 197
13 Assessment of Endothelial Damage/Dysfunction: A Focus on Circulating Endothelial Cells Christopher J. Boos Andrew D. Blann Gregory Y. H. Lip 211
14 Evaluation of Endothelial Function by In Vivo Microscopy Rosario Scalia 225
15 Haptotaxis of Endothelial Cell Migration Under Flow Steve Hsu Rahul Thakar Song Li 237
16 Isolation, Culture, and Functional Analysis ofAdult Mouse Cardiomyocytes Ronglih Liao Mohit Jain 251
17 Mechanical Measurement of Contractile Function of Isolated Ventricular Myocytes Loren E. Wold Jun Ren 263
18 Studying Ischemia and Reperfusion in Isolated Neonatal Rat Ventricular Myocytes Using Coverslip Hypoxia Kelly R. Pitts Christopher F. Toombs 271
19 Isolation and Functional Studies of Rat Aortic Smooth Muscle Cells Nair Sreejayan Xiaoping Yang 283
20 Detection of Reactive Oxygen Species and Nitric Oxide in Vascular Cells and Tissues Hua Cai Sergey Dikalov Kathy K. Griendling David G. Harrison 293
21 Assessment of Protein Glycoxidation in Ventricular Tissues Shi-Yan Li Jun Ren 313
22 Assessment of PI-3 Kinase and Akt in Ischemic Heart Diseases in Diabetes Takashi Matsui Amy J. Davidoff 329
23 Two-Dimensional Gel Electrophoresis in Platelet Proteomics Research Angel Garcia 339
24 Stem Cell Therapy in the Heart and Vasculature Loren E. Wold Wangde Dai Joan S. Dow Robert A. Kloner 355
Index 367
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