Plant Cell Culture Protocols Book

Part I. Introduction

An Introduction to Plant Cell Culture: Back to the Future

Victor M. Loyola-Vargas and Felipe Vázquez-Flota

History of Plant Tissue Culture

Trevor A. Thorpe

Part II. Cell Culture and Plant Regeneration: The Fundamentals

Pathogen and Biological Contamination Management: The Road Ahead

Alan C. Cassells and Barbara M. Doyle

Growth Measurements: Estimation of Cell Division and Cell Expansion

Gregorio Godoy-Hernández and Felipe A. Vázquez-Flota

Callus and Suspension Culture Induction, Maintenance, and Characterization

Alejandrina Robledo-Paz, María Nélida, Vázquez-Sánchez, Rosa María Adame-Alvarez, and Alba Estela Jofre-Garfias

Measurement of Cell Viability in In Vitro Cultures

Lizbeth A. Castro-Concha, Rosa María Escobedo, and María de Lourdes Miranda-Ham

Cryopreservation of Embryogenic Cell Suspensions by Encapsulation-Vitrification

Qiaochun Wang and Avihai Perl

Somatic Embryogenesis in Picea Suspension Cultures

Claudio Stasolla

Indirect Somatic Embryogenesis in Cassava for Genetic Modification Purposes

Krit Raemakers, Isolde Pereira, Herma Koehorst van Putten, and Richard Visser

Direct Somatic Embryogenesis in Coffea canephora

Francisco Quiroz-Figueroa, Miriam Monforte-González, Rosa M. Galaz-Ávalos, and Victor M. Loyola-Vargas

Part III. Plant Propagation In Vitro

A New Temporary Immersion Bioreactor System for Micropropagation

Manuel L. Robert, José Luis Herrera-Herrera, Gastón Herrera-Herrera, Miguel Ángel Herrera-Alamillo, and Pedro Fuentes-Carrillo

Protocol to Achieve Photoautotrophic Coconut Plants Cultured In Vitro With Improved Performance Ex Vitro

Gabriela Fuentes, Carlos Talavera, Yves Desjardins, and Jorge M. Santamaría

Use of Statistics in Plant Biotechnology

Michael E. Compton

An Efficient Method for the Micropropagation of Agave Species

Manuel L. Robert, José Luis Herrera-Herrera, Eduardo Castillo, Gabriel Ojeda, and Miguel Angel Herrera-Alamillo

Micropropagation of Endangered Plant Species

Zhihua Liao, Min Chen, Xiaofen Sun, and Kexuan Tang

Clonal Propagation of Softwoods

Trevor A. Thorpe, Indra S. Harry, and Edward C. Yeung

Part IV. Applications for Plant Protoplasts

Isolation, Culture, and Plant Regeneration From Leaf Protoplasts of Passiflora

Michael R. Davey, Paul Anthony, J. Brian Power, and Kenneth C. Lowe

Isolation, Culture, and Plant Regeneration From Echinacea purpurea Protoplasts

Zeng-guang Pan, Chun-zhao Liu, Susan J. Murch, and Praveen K. Saxena

Production of Cybrids in Brassicaceae Species

Maksym Vasylenko, Olga Ovcharenko, Yuri Gleba, and Nikolay Kuchuk

Guard Cell Protoplasts: Isolation, Culture, and Regeneration of Plants

Gary Tallman

Production of Interspecific Hybrid Plants in Primula

Juntaro Kato and Masahiro Mii

Part V. Protocols for Genomic Manipulation

Agrobacterium-Mediated Transformation of Petunia Leaf Discs

Ingrid M. van der Meer

Transformation of Wheat Via Particle Bombardment

Indra K. Vasil and Vilma Vasil

Chloroplast Transformation

Xiao-Mei Lu, Wei-Bo Yin, and Zan-Min Hu

The Biochemical Basis for the Resistance to Aluminum and Their Potential as Selection Markers

César De los Santos-Briones and Teresa Hernández-Sotomayor

Transformation of Maize Via Agrobacterium tumefaciens Using a Binary Co-Integrate Vector System

Zuo-yu Zhao and Jerry Ranch

Part VI. Accumulation of Metabolites in Plant Cells

Capsaicin Accumulation in Capsicum spp. Suspension Cultures

Neftalí Ochoa-Alejo

Isolation and Purification of Ribosome-Inactivating Proteins

Sang-Wook Park, Balakrishnan Prithiviraj, Ramarao Vepachedu, and Jorge M. Vivanco

Catharanthus roseus Shoot Cultures for the Production of Monoterpenoid Indole Alkaloids

Elizabeta Hernández-Domínguez, Freddy Campos-Tamayo, Mildred Carrillo-Pech, and Felipe Vázquez-Flota

Methods for Regeneration and Transformation in Eschscholzia californica: A Model Plant to Investigate Alkaloid Biosynthesis

Benjamin P. MacLeod and Peter J. Facchini

Appendix A: The Components of the Culture Media

Appendix B: Plant Biotechnology and Tissue Culture Resources on the Internet

Index