DNA Repair Protocols Book

Isolation of Mutagen-Sensitive Chinese Hamster Cell Lines by Replica Plating

Malgorzata Z. Zdzienicka

Complementation Assays Adapted for DNA Repair-Deficient Keratinocytes

Mathilde Fréchet, Valérie Bergoglio, Odile Chevallier-Lagente, Alain Sarasin, and Thierry Magnaldo

Cytogenetic Challenge Assays for Assessment of DNA Repair Capacities

William W. Au and Salama A. Salama

Evaluating the Delayed Effects of Cellular Exposure to Ionizing Radiation

Shruti Nagar, James J. Corcoran, and William F. Morgan

Inhibition of DNA Synthesis by Ionizing Radiation: A Marker for an S-Phase Checkpoint

Nicolaas G. J. Jaspers and Malgorzata Z. Zdzienicka

Analysis of Inhibition of DNA Replication in Irradiated Cells Using the SV40-Based In Vitro Assay of DNA Replication

George Iliakis, Ya Wang, and Hong Yan Wang

Cytometric Assessment of Histone H2AX Phosphorylation: A Reporter of DNA Damage

Xuan Huang and Zbigniew Darzynkiewicz

Detection of DNA Strand Breaks by Flow and Laser Scanning Cytometry in Studies of Apoptosis and Cell Proliferation (DNA Replication)

Zbigniew Darzynkiewicz, Xuan Huang, and Masaki Okafugi

In Vitro Rejoining of Double-Strand Breaks in Genomic DNA

George Iliakis and Nge Cheong

Detection of DNA Double-Strand Breaks and Chromosome Translocations Using Ligation-Mediated PCR and Inverse PCR

Michael J. Villalobos, Christopher J. Betti, and Andrew T. M Vaughan

Plasmid-Based Assays for DNA End-Joining In Vitro

George Iliakis, Bustanur Rosidi, Minli Wang, and Huichen Wang

Use of Gene Targeting to Study Recombination in Mammalian Cell DNA Repair Mutants

Rodney S. Nairn and Gerald M. Adair

Gene-Specific and Mitochondrial Repair of Oxidative DNA Damage

R. Michael Anson, Penelope A. Mason, and Vilhelm A. Bohr

Quantitative PCR-Based Measurement of Nuclear and Mitochondrial DNA Damage and Repair in Mammalian Cells

Janine H. Santos, Joel N. Meyer, Bhaskar S. Mandavilli, and Bennett Van Houten

Measuring the Formation and Repair of DNA Damage by Ligation-Mediated PCR

Gerd P. Pfeifer

Immunochemical Detection of UV-Induced DNA Damage and Repair

Marcus S. Cooke and Alistair Robson

A Dot-Blot Immunoassay for Measuring Repair of Ultraviolet Photoproducts

Shirley McCready

Quantification of Photoproducts in Mammalian Cell DNA Using Radioimmunoassay

David L. Mitchell

DNA Damage Quantitation by Alkaline Gel Electrophoresis

Betsy M. Sutherland, Paula V. Bennett, and John C. Sutherland

The Comet Assay: A Sensitive Genotoxicity Test for the Detection of DNA Damage and Repair

Günter Speit and Andreas Hartmann

Fast Micromethod DNA Single-Strand-Break Assay

Heinz C. Schröder, Renato Batel, Heiko Schwertner, Oleksandra Boreiko, and Werner E. G. Müller

32P-Postlabeling DNA Damage Assays: PAGE, TLC, and HPLC

Shinya Shibutani, Sung Yeon Kim, and Naomi Suzuki

Electrophoretic Mobility Shift Assays to Study Protein Binding to Damaged DNA

Vaughn Smider, Byung Joon Hwang, and Gilbert Chu

Construction of MMR Plasmid Substrates and Analysis of MMR Error Correction and Excision

Huixian Wang and John B. Hays

Characterization of Enzymes that Initiate Base Excision Repair at Abasic Sites

Walter A. Deutsch and Vijay Hegde

Base Excision Repair in Mammalian Cells

Yoshihiro Matsumoto

In Vitro Base Excision Repair Assay Using Mammalian Cell Extracts

Guido Frosina, Enrico Cappelli, Monica Ropolo, Paola Fortini, Barbara Pascucci, and Eugenia Dogliotti

Biochemical Assays for the Characterization of DNA Helicases

Robert M. Brosh, Jr. and Sudha Sharma

Repair Synthesis Assay for Nucleotide Excision Repair Activity Using Fractionated Cell Extracts and UV-Damaged Plasmid DNA

Maureen Biggerstaff and Richard D. Wood

Assaying for the Dual Incisions of Nucleotide Excision Repair Using DNA with a Lesion at a Specific Site

Mahmud K. K. Shivji, Jonathan G. Moggs, Isao Kuraoka, and Richard D. Wood

Analysis of Proliferating Cell Nuclear Antigen (PCNA) Associated with DNA Excision Repair Sites in Mammalian Cells

A. Ivana Scovassi and Ennio Prosperi

Analysis of DNA Repair and Chromatin Assembly In Vitro Using Immobilized Damaged DNA Substrates

Jill A. Mello, Jonathan G. Moggs, and Geneviève Almouzni

Index