Human Embryonic Stem Cell Protocols Book

1. Human Embryo Culture and Assessment for the Derivation of Embryonic Stem Cells-ESC

A. Henry Sathananthan and Tiki Osianlis

2. Derivation of Human Embryonic Stem Cell Lines from Vitrified Human Embryos

Teija T. Peura, Julia Schaft, and Tomas Stojanov

3. Growth of Human Embryonic Stem Cells Using Derivates of Human Fibroblasts

Carmen Escobedo-Lucea and Miodrag Stojkovic

4. In Vitro Neural Differentiation of Human Embryonic Stem Cells Using a Low-Density Mouse Embryonic Fibroblast Feeder Prool

John A. Ozolek, Esther P. Jane, James E. Esplen, Patti Petrosko, Amy K. Wehn, Teresa M. Erb, Sara E. Mucko, Lyn C. Cote, and Paul J. Sammak

5. Optimization of Physiological Xenofree Molecularly-Defined Media and Matrices to Maintain Human Embryonic Stem Cell Pluripotency

Isabelle Peiffer, Romain Barbet, Antoinette Hatzfeld, Ma-Lin Li, and Jacques A. Hatzfeld

6. Serum-Free and Feeder-Free Culture Expansion of Human Embryonic Stem Cells

Katherine E. Wagner and Mohan C. Vemuri

7. Single Cell Enzymatic Dissociation of Human Embryonic Stem Cells: A Straightforward, Robust, and Standardized Culture Method

Catharina Ellerström, Johan Hyllner, and Raimund Strehl

8. Monitoring Stemness in Long-Term hESC Cultures by Real-Time PCR

Amparo Galán and Carlos Simón

9. Culture and Preparation of Human Embryonic Stem Cells for Proteomics-Based Applications

Charles C. King

10. A Two- and Three-Dimensional Approach for Visualizing Human Embryonic Stem Cell Differentiation

Christian B. Brøchner, Peter S. Vestentoft, Niels Lynnerup, Claus Yding Andersen, and Kjeld Møllgård

11. Immunoflourescence and mRNA Analysis of Human Embryonic Stem Cells (hESCs) Grown Under Feeder-Free Conditions

Aashir Awan, Roberto S. Oliveri, Pernille L. Jensen, Søren T. Christensen, and Claus Yding Andersen

12. Study of Gap Junctions in Human Embryonic Stem Cells

Raymond C.B. Wong and Alice Pébay

13. hESC Engineering by Integrase-Mediated Chromosomal Targeting

Ying Liu, Uma Lakshmipathy, Ali Ozgenc, Bhaskar Thyagarajan, Pauline Lieu, Andrew Fontes, Haipeng Xue, Kelly Sheyhing, Chad MacArthur, and Jonathan D. Chesnut

14. Human Embryonic Stem Cell Differentiation on Periodontal Ligament Fibroblasts

Y. Murat Elçin, Bülend Inanç, and A. Eser Elçin

15. Generation of Neural Crest Cells and Peripheral Sensory Neurons from Human Embryonic Stem Cells

Ronald S. Goldstein, Oz Pomp, Irina Brokhman, and Lina Ziegler

16. Embryonic Stem Cells as a Model for Studying Melanocyte Development

Susan E. Zabierowski and Meenhard Herlyn

17. In Vitro Derivation of Chondrogenic Cells from Human Embryonic Stem Cells

Wei Seong Toh, Eng Hin Lee, Mark Richards, and Tong Cao

18. Vascular Differentiation of Human Embryonic Stem Cells in Bioactive Hydrogel-Based Scaffolds

Sharon Gerecht, Lino S. Ferreira, and Robert Langer

19. Differentiation of Neural Precursors and Dopaminergic Neurons from Human Embryonic Stem Cells

Xiao-Qing Zhang and Su-Chun Zhang

20. Transplantation of Human Embryonic Stem Cells and Derivatives to the Chick Embryo

Ronald S. Goldstein

21. Genetic Manipulation of Human Embryonic Stem Cells

Silvina Epsztejn-Litman and Rachel Eiges

22. Genetic Manipulation of Human Embryonic Stem Cells in Serum and Feeder Free Media

Stefan R. Braam, Chris Denning, and Christine L. Mummery

23. Human Induced Pluripotent Stem Cells: Derivation, Propagation, and Freezing in Serum- and Feeder Layer-Free Culture Conditions

Hossein Baharvand, Mehdi Totonchi, Adeleh Taei, Ali Seifinejad, Nasser Aghdami, and Ghasem Hosseini Salekdeh