Molecular Diagnosis of Cancer: Methods and Protocols Book

	Preface
	Contributors
1	Prognostic and predictive factors	1
2	Assessment of predictive values of tumor markers	13
3	Quality assurance of predictive markers in breast cancer	29
4	Extraction of nucleic acid templates	59
5	Microdissection and extraction of DNA from archival tissue	71
6	Fluorescence in situ hybridization : technical overview	77
7	HER2 FISH in breast cancer	89
8	Fluorescence in situ hybridization for BCR-ABL	103
9	UroVysion multiprobe FISH in urinary cytology	117
10	Chromogenic in situ hybridization in tumor pathology	133
11	Comparative genomic hybridization and fluorescence in situ hybridization in chronic lymphocytic leukemia	145
12	Molecular characterization of human papillomaviruses by PCR and in situ hybridization	159
13	A nested RT-PCR assay to detect BCR/abl	181
14	TP53 mutation detection by SSCP and sequencing	191
15	PCR diagnosis of T-cell lymphoma in paraffin-embedded bone marrow biopsies	209
16	Circulating DNA analysis : protocols and clinical applications using taqman assays	217
17	Microsatellite instability : theory and methods	237
18	The diagnostic and prognostic significance of the methylation status of Myf-3 in lymphoproliferative disorders	251
19	Quantitative analysis of PRAME for detection of minimal residual disease in leukemia	267
20	Determination of cyclin D1 expression by quantitative real-time, reverse-transcriptase polymerase chain reaction	277
21	Detection of telomerase hTERT gene expression and its splice variants by RT-PCR	297
22	Detection of telomerase enzyme activity by TRAP assay	311
23	Identification of TP53 mutations in human cancers using oligonucleotide microarrays	323
24	Detection of K-ras mutations by a microelectronic DNA chip	337
25	Microarray-based CGH in cancer	355
26	Tissue microarrays	377
	Index	391