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Preface | v | |
Contributors | ix | |
1 | Identification and Analysis of Precursors to Invasive Pancreatic Cancer | 1 |
2 | Optimal Molecular Profiling of Tissue and Tissue Components: Defining the Best Processing and Microdissection Methods for Biomedical Applications | 15 |
3 | Immunohistochemistry and In Situ Hybridization in Pancreatic Neoplasia | 67 |
4 | Practical Methods for Tissue Microarray Construction | 89 |
5 | Xenografting and Harvesting Human Ductal Pancreatic Adenocarcinomas for DNA Analysis | 103 |
6 | Culture and Immortalization of Pancreatic Ductal Epithelial Cells | 113 |
7 | DNA Methylation Analysis in Human Cancer | 123 |
8 | Digital Single-Nucleotide Polymorphism Analysis for Allelic Imbalance | 137 |
9 | Representational Difference Analysis as a Tool in the Search for New Tumor Suppressor Genes | 143 |
10 | Serial Analyses of Gene Expression (SAGE) | 161 |
11 | Oligonucleotide-Directed Microarray Gene Profiling of Pancreatic Adenocarcinoma | 175 |
12 | Identification of Differentially Expressed Proteins in Pancreatic Cancer Using a Global Proteomic Approach | 189 |
13 | Detection of Telomerase Activity in Patients with Pancreatic Cancer | 199 |
14 | Serological Analysis of Expression cDNA Libraries (SEREX): An Immunoscreening Technique for Identifying Immunogenic Tumor Antigens | 207 |
15 | Modeling Pancreatic Cancer in Animals to Address Specific Hypotheses | 217 |
16 | Strategies for the Use of Site-Specific Recombinases in Genome Engineering | 245 |
17 | Primary Explant Cultures of Adult and Embryonic Pancreas | 259 |
18 | Zebrafish as a Model for Pancreatic Cancer Research | 273 |
19 | Development of a Cytokine-Modified Allogeneic Whole Cell Pancreatic Cancer Vaccine | 299 |
20 | Overview of Linkage Analysis: Application to Pancreatic Cancer | 329 |
Index | 343 |
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