Platelets and Megakaryocytes: Volume 1: Functional Assays Book

1	Effects of anticoagulants used during blood collection on human platelet function	3
2	Preparation of washed platelet suspensions from human and rodent blood	13
3	Platelet counting	29
4	Electron microscopy methods for studying platelet structure and function	47
5	Platelet aggregation : turbidimetric measurements	65
6	Platelet aggregation in whole blood : impedance and particle counting methods	77
7	Secretion from dense granules : luminescence method for adenine nucleotides	89
8	Platelet dense-granule secretion : the [[superscript 3]H]-5-HT secretion assay	95
9	Analysis of releasable nucleotides of platelets	97
10	Studies of secretion using permeabilized platelets	109
11	Measurement of platelet arachidonic acid metabolism	121
12	Measurement of the platelet procoagulant response	135
13	Platelet adhesion assays performed under static conditions	145
14	Platelet adhesion assays under flow using matrix protein-coupled adhesion columns	153
15	Techniques to examine platelet adhesive interactions under flow	165
16	In vivo models of platelet function and thrombosis : study of real-time thrombus formation	187
17	Adhesion between platelets and leukocytes or endothelial cells	199
18	In vitro measurement of high-shear platelet adhesion and aggregation by the PFA-100	215
19	Flow-cytometric analysis of platelet-membrane glycoprotein expression and platelet activation	225
20	Flow-cytometric analysis of mouse platelet function	255
21	Platelet microparticles	269
22	Isolation of primary megakaryocytes and studies of proplatelet formation	281
23	Isolation and culture of megakaryocyte precursors	293
24	Assays of megakaryocyte development : surface antigen expression, ploidy, and size	309
25	Assays of megakaryocytes development : cytoplasm, storage granules, and demarcation membranes	323
26	Thrombopoietin bioassay	347
27	Culture of megakaryocytic cell lines : uses and limitations	361