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Part I. Design and Computational Strategies for Protein Engineering
Combinatorial Protein Design Strategies Using Computational Methods
Hidetoshi Kono, Wei Wang, and Jeffery G. Saven
Global Incorporation of Unnatural Amino Acids in Escherichia coli
Jamie M. Bacher and Andrew D. Ellington
Considerations in the Design and Optimization of Coiled Coil Structures
Jody M. Mason, Kristian M. Müller, and Katja M. Arndt
Calcium Indicators Based on Calmodulin-Fluorescent Protein Fusions
Kevin Truong, Asako Sawano, Atsushi Miyawaki, and Mitsuhiko Ikura
Design and Synthesis of Artificial Zinc Finger Proteins
Wataru Nomura and Yukio Sugiura
Monobodies: Antibody Mimics Based on the Scaffold of the Fibronectin Type III Domain
Akiko Koide and Shohei Koide
Engineering Site-Specific Endonucleases
Peter Friedhoff and Alfred Pingoud
Part II. Evolutionary Strategies for Protein Engineering
Protein Library Design and Screening: Working Out the Probabilities
Michel Denault and Joelle N. Pelletier
Protein Design by Binary Patterning of Polar and Nonpolar Amino Acids
Luke H. Bradley, Yinan Wei, Peter Thumfort, Christine Wurth, and Michael H. Hecht
Versatile DNA Fragmentation and Directed Evolution With Nucleotide Exchange and Excision Technology
Sabine C. Stebel, Katja M. Arndt, and Kristian M. Müller
Degenerate Oligonucleotide Gene Shuffling
Peter L. Bergquist and Moreland D. Gibbs.
M13 Bacteriophage Coat Proteins Engineered for Improved Phage Display
Sachdev S. Sidhu, Birte K. Feld, and Gregory A. Weiss
Ribosome-Inactivation Display System
Satoshi Fujita, Jing-Min Zhou, and Kazunari Taira
Compartmentalized Self-Replication: A Novel Method for the Directed Evolution of Polymerases and Other Enzymes
Farid J. Ghadessy and Philipp Holliger
Synthesis of Degenerated Libraries of the Ras-Binding Domain of Raf and Rapid Selection of Fast-Folding and Stable Clones With the Dihydrofolate Reductase Protein Fragment Complementation Assay
François-Xavier Campbell-Valois and Stephen W. Michnick
A General Method of Terminal Truncation, Evolution, and Re-Elongation to Generate Enzymes of Enhanced Stability
Jochen Hecky, Jody M. Mason, Katja M. Arndt, and Kristian M. Müller
Index
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