PCR Detection of Microbial Pathogens Book

	Preface
	Contributors
Pt. I	Reviews
1	Specificity and Performance of Diagnostic PCR Assays	3
2	Pre-PCR Processing of Samples	31
3	Critical Aspects of Standardization of PCR	51
4	PCR Technology and Applications to Zoonotic Food-Borne Bacterial Pathogens	65
Pt. II	Protocols
5	Detection, Identification, and Subtyping of Actinobacillus pleuropneumoniae	87
6	Identification and Differentiation of Brucella abortus Field and Vaccine Strains by BaSS-PCR	97
7	Isolation of Campylobacter and Identification by PCR	109
8	Detection of Differentiation of Chlamydiae by Nested PCR	123
9	Detection of Toxigenic Clostridia	137
10	PCR-Based Detection of Coxiella burnetii from Clinical Samples	153
11	Detection and Subtyping of Shiga Toxin-Producing Escherichia coli (STEC)	163
12	Detection of Listeria monocytogenes Using a PCR/DNA Probe Assay	185
13	Detection of Leptospira interrogans	193
14	Detection of Pathogenic Mycobacteria of Veterinary Importance	201
15	Multiplex PCR of Avian Pathogenic Mycoplasmas	223
16	Detection of Differentiation of Ruminant Mycoplasmas	231
17	Detection of Mycoplasmas hyopneumoniae from Clinical Samples and Air	247
18	PCR-Detection of Hemophilus paragallinarum, Hemophilus somnus, Mannheimia (Pasteurella) hemolytica, Mannheimia spp., Pasteurella trehalosi, and Pasteurella multocida	257
19	Detection of Salmonella spp.	275
20	Detection of Toxoplasma gondii	289
21	PCR-Derived Methods for the Identification of Trichinella Parasites from Animal and Human Samples	299
22	Detection of Pathogenic Yersinia enterocolitica by a Swab Enrichment PCR Procedure	311
	Index	325