Biomedical EPR - Part a: Free Radicals, Metals, Medicine and Physiology Book

Section I. Instrumentation and Methodology

Chapter 1

Saturation Recovery EPR; Sandra S. Eaton and Gareth R. Eaton

1. Motivation

2. Brief History

3. Information Content of Saturation Recovery Curves

4. Practical Aspects of Experimental Methodology

5. Applications

6. Prognosis

7. References

Chapter 2

Loop-Gap Resonators; George A. Rinard and Gareth R. Eaton

1. Introduction

2. History

3. Why should one use loop-gap resonators?

4. Basics

5. Topologies of loop gap resonators

6. Coupling to Resonators

7. Design equations

8. Magnetic Field Modulation

9. LGR for Time Domain EPR

10. Selection of the Q of a LGR

11. Measuring B1 in the LGR

12. Variable Temperature

13. Mechanical Considerations

14. Commercial Resonators

15. Applications of Lumped-Circuit Resonators

16. Further information

17. References

Chapter 3

EPR Interfaced To Rapid Mixing; Charles P. Scholes

1. Introduction

2. The Loop Gap Resonator Based Stopped-Flow System

3. Dielectric Resonator-based Stopped-Flow EPR

4. Applications of Stopped-Flow and Flow EPR to Naturally Occurring Transient Radicals

5. Future Developments and Applications of Flow and Stopped-Flow EPR

6. References

Chapter 4

Application of Angle-Selected Electron Nuclear Double Resonance to Characterize Structured Solvent in Small Molecules and Macromolecules; Devkumar Mustafi and Marvin W. Makinen

1. Introduction

2. ENDOR Assignment of Molecular Structure and Conformation with VO2+ and Nitroxyl Spin-Labels

3. ENDOR Characterization of Structured Solvent in Small Molecule Complexes and in Proteins

4. Future Perspectives and Concluding Remarks

5. References

Chapter 5

Solution-ENDOR of Some Biologically Interesting

Radical Ions; Fabian Gerson and Georg Gescheidt

1. Solution ENDOR Spectroscopy

2. Quinones

3. Porphyrinoids

4. References

Chapter 6

Electron-Electron Double Resonance; Lowell D. Kispert

1. Introduction

2. Instrumental Techniques

3. Dynamics of Biomolecules in Liquid Crystals, Glassy Solids, Polymers and Crystals

4. Practical Aspects of Measurements

5. References

Chapter 7

Digital Detection by Time-Locked Sampling in EPR; James S. Hyde, Theodore G. Camenisch, Joseph J. Ratke, Robert A. Strangeway, Wojciech Froncisz

1. Introduction

2. Time Locking and Superheterodyne Detection—EPR Instrument Design Background

3. Time-Locked Subsampling Detection for CW EPR

4. Pulse Saturation Recovery Using Time-Locked Subsampling

5. Selected Engineering Considerations

6. Conclusion

7. References

Chapter 8

Measurement of Distances Between Electron Spins Using Pulsed EPR; Sandra S. Eaton and Gareth R. Eaton

1. Introduction

2. Fundamental Principles of Interaction between Electron Spins

3. Distance between Two Slowly Relaxing Centers

4. Distance between a Slowly Relaxing Center and a Rapidly-Relaxing Center

5. Some Practical Considerations

6. Recent Examples for Distances between Two Slowly-Relaxing Radicals

7. Recent Examples for Distances between a Rapidly-Relaxing and a Slowly-Relaxing Spin

8. Prognosis

9. References

Section II. Motion, Proteins, and Membranes

Chapter 9

ESR and Molecular Dynamics; Jack H. Freed

1. Motional Narrowing and Organic Radicals

2. Double Resonance and Molecular Dynamics

3. Slow Motional ESR and Molecular Dynamics

4. High Field ESR and Molecular Dynamics

5. Spin-Echoes and Molecular Dynamics

6. Two-Dimensional Fourier Transform ESR

7. Prospectus

8. Glossary of Abbreviations

9. References

Chapter 10

SDSL: A Survey of Biological Applications; Candice S. Klug and Jimmy B. Feix

1. Introduction

2. Solvent accessibility

3. Motion

4. Distance measurements

5. Methodology

6. Conclusion

7. References

Chapter 11

Saturation Transfer Spectroscopy of Biological Membranes; Derek Marsh, László I. Horváth, Tibor Páli And Vsevolod A. Livshits

1. Introduction

2. Historical Development

3. Rapid-Passage Saturation-Transfer-EPR Displays

4. Modulation-Coupled Bloch Equations

5. Slow Rotational Diffusion

6. Applications: Slow Rotation

7. T1-Sensitive Nonlinear EPR Displays

8. Slow Exchange and Paramagnetic Enhancements

9. Applications: Relaxation Enhancements

10. Outlook

11. References

Chapter 12

Saturation Transfer EPR: Rotational Dynamics of Membrane Proteins; Albert H. Beth and Eric J. Hustedt

1. Introduction

2. Methods for Analysis of ST-EPR Data

3. Overview of Theory for Calculation of ST-EPR Spectra

4. Nonlinear Least Squares Methods of Data Analysis

5. Model Calculations of ST-EPR Spectra Using the Transition Rate Matrix Approach

6. Applications of ST-EPR to Membrane Proteins

7. References

Chapter 13

Trends in EPR Technology; James S. Hyde

1. Introduction

2. Resonators

3. Noise

4. Multifrequency EPR

5. EPR for Routine Analysis

6. Discussion

7. References

Chapter 14

Prognosis; Sandra S. Eaton and Gareth R. Eaton

Contents of Previous Volumes

Index